[Histonet] Helicobacter pylori immunocytochemistry - cytoplasmatic
gu.lang <@t> gmx.at
Tue Jan 15 14:56:04 CST 2013
I need some help with the interpretation of Hp-IHC. I stained several cases
of FFPE gastric biopsies with polyclonal anti Hp from Cell Marque (1:200,
Some cases showed the nice bacteria and had usually a clean overall
background. But some cases showed no bacteria but slight cellular staining
of epithelial cells.
It was confined on the cells, and usually not all cells in the biopsy had
>From literature I learned, that Hp brings its toxine and other proteins
(CagA) into the hostcell. Is it possible, that the polyclonal antibody
detects this stuff, although hardly an intact bacterium can be seen?
Anybody with similar findings?
Thanks in advance
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