[Histonet] Re: tricks about antibody generated in human on human

Hobbs, Carl carl.hobbs <@t> kcl.ac.uk
Fri Jan 11 14:00:18 CST 2013

Good advice given.

Biotinylated primaries to be followed by stAvidin-488 or stABCpx-DAB, for eg.
Or, if biotinylated primary gives good result with above but, at low dilution factor,  use tyramide amplification?

Human- on- human can be just fine, depending on what proteins you want to demonstrate and, in what tissue.
What tissue are you working on?
What tissue prep ( Pwax/frozen)?
You  using patients' serum containing auto-antibodies?

Or, do you have a human that you are injecting human Ags into, then bleeding that person so you can harvest Abs?


NB: You suggested diluting out your secondary...not good as you will just lower your specific signal as well, imho.

Curious Carl

Carl Hobbs
Histology Manager
Wolfson CARD
School of Biomedical Sciences
Kings College London
Guys Campus
Tel: 020 78486813
Fax: 020 78486816
020 78486813

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