[Histonet] Re: tricks about antibody generated in human on human
carl.hobbs <@t> kcl.ac.uk
Fri Jan 11 14:00:18 CST 2013
Good advice given.
Biotinylated primaries to be followed by stAvidin-488 or stABCpx-DAB, for eg.
Or, if biotinylated primary gives good result with above but, at low dilution factor, use tyramide amplification?
Human- on- human can be just fine, depending on what proteins you want to demonstrate and, in what tissue.
What tissue are you working on?
What tissue prep ( Pwax/frozen)?
You using patients' serum containing auto-antibodies?
Or, do you have a human that you are injecting human Ags into, then bleeding that person so you can harvest Abs?
NB: You suggested diluting out your secondary...not good as you will just lower your specific signal as well, imho.
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