[Histonet] staining site of bone deposition on live specimens

Christopher Sheil csheil <@t> jcu.edu
Mon Jan 7 09:17:03 CST 2013

Earlier I submitted a question to histonet to ask about labels that  
can be used to bind bone at the site of bone formation in aquatic  
amphibians.  Robert Schoonhoven kindly sent a response stating that  
tetracycline has been used in mammalian systems.  I also recently  
found a paper (citation below) that outlines the use of tetracycline,  
calcein, xylenol orange, and alizarine red S to identify Layers of  
Active Growth (LAG's) in a primarily aquatic frog.

Francillon H and J Castanet. 1985. Experimental demonstration of the  
annual characteristic of the lines of arrest of skeletal growth in  
Rana esculenta (Amphibia, Anura). Comptes Rendus de L'Academie des  
Sciences, Serie III, Sciences de la vie. 300(8): 327–332.


Christopher Sheil
Graduate Coordinator
Department of Biology
John Carroll University
20700 North Park Boulevard
University Heights, OH  44118
Email: csheil <@t> jcu.edu
Phone: 216.397.3088

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