[Histonet] RESCUING UNDER DECALCIFIED BONE FROM PARAFFIN EMBEDDED BLOCKS

[Rene J Buesa]

Go back to water (xylene or whatever "clearant" you use → graded alcohols → water) and place in decal.
The results will be slightly of less quality than if the tissue was correctly decalled in the first place, but you will be able to section it.
René J. 

From: Fiona J Morrow <f.morrow <@t> sheffield.ac.uk>
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Thursday, February 28, 2013 8:59 AM
Subject: [Histonet] RESCUING UNDER DECALCIFIED BONE FROM PARAFFIN EMBEDDED BLOCKS

Hi

Has anyone ever tried to reprocess bone tissue that has been under
decalcified and processed through to wax?

Thanks

Fi M


Fiona Morrow
Dept. of Infection and Immunity
KFloor, Room K118
Medical School
University of Sheffield
Beech Hill Road
Sheffield
S10 2RX

0114 271 2102
F.morrow <@t> sheffield.ac.uk

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