[Histonet] Alcian Blue

[Smith, Allen]

Alcian blue lasts until loss of dye onto the sections weakens staining.  If one runs many slides through it, it may not last 6 weeks.  With light use, it may last 6 years.
Allen A. Smith
Professor of Anatomy
Barry University School of Podiatric Medicine

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala
Sent: Wednesday, February 27, 2013 3:03 PM
To: Rene J Buesa; Diana McCaig; 'histonet <@t> lists.utsouthwestern.edu'
Subject: RE: [Histonet] Alcian Blue

Why would you use a reagent until it starts to weaken, meaning the staining intensity and results are changing over time.  In my opinion that's really poor quality control.  Stain intensity, specificity and sensitivity should remain constant.

Liz

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, February 27, 2013 11:57 AM
To: Diana McCaig; 'histonet <@t> lists.utsouthwestern.edu'
Subject: Re: [Histonet] Alcian Blue

Until the staining starts to weaken
René J.

From: Diana McCaig <dmccaig <@t> ckha.on.ca>
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, February 27, 2013 2:55 PM
Subject: [Histonet] Alcian Blue

What is the shelf life of  prepared  1% Alcian blue in 3% Acetic Acid?

Diana
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