[Histonet] IgG4

[Sue Hunter]

We use Cell Marques Mouse monoclonal (clone MRQ-44)  Cat# 367M-16 at a 1:200 dilution on the Ultra. We are using the Optiview detection kits.
CC#1-24 min
Option 5 - 12 min
Antibody - 24  min  
OV Linker 8 min
Multimer 8 min
H2O2 +DAB 8 min
OV copper 4 min
Hematozylin II  8min
bluing  4 min

We only test FFPE tissues.
Sue

Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shunter <@t> beaumont.edu


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Susan Foreman
Sent: Monday, February 25, 2013 2:57 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IgG4

Has anyone worked up IgG4 on the Ventana Benchmark Ultra?  What vendor?  

 

How about running it on fresh frozen sections?  FITC?  Vendor?

 

Many Thanks for your input,

Susan

 

 

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