[Histonet] Question for Alkaline Phosphatase / Biotin System Users

[Amy Porter]

We are using standard avidin / biotin staining with alkaline phosphatase
labeling and sometimes, not every time and not every slide in the same run
we get residual blotchy spots and streaking of the reaction on and off the
tissue.  We have increased to two rinses after primary and two rinses after
alk phos reagent prior to substrate; still happening on some slides...anyone
else have this experience or type of problem?  Any experiences or
suggestions are appreciated.  Just can't figure out what is causing it..

 

Amy S. Porter, HT(ASCP) QIHC

Michigan State University

Investigative HistoPathology Laboratory

portera <@t> msu.edu