[Histonet] FISH signals

IRENA SREBOTNIK KIRBIS irena.kirbis <@t> hotmail.com
Sat Feb 16 08:58:48 CST 2013


Hi,
 how the FISH signals in FFPE tissue can be strenghten? by standard procedure only weak signals for EGFR can be obtained, so far I tried different time in Vysis protease 1 (45, 60, 70 min), for other probes 70 min works quite well on our FFPE, but not for EGFR? what other modifications can I try?
many thanks
Irena 		 	   		  


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