[Histonet] RE: Type I Water

[Akemi Allison]

It is recommended to use DI water for your water bath, especially when cutting tissues for IHC.  Tap water has all kinds of bacteria, as well as potential chorine, rust, lead and other components which might have adverse affects to your histologic testing.  Ethel Macrea from Ventana has presented an excellent workshop on water for use in the histology lab at NSH national and state meetings.  If you have an opportunity i highly recommend attending  As a good housekeeping practice, I always recommend cleaning your water baths with a soapy detergent after use, rinsing with tap water, drying residual water from the water bath and either turning upside down, or covering to prevent dust particles.
 
Akemi Allison BS, HT(ASCP)HTL






________________________________
 From: Sarah Dysart <sdysart <@t> mirnarx.com>
To: Nancy Schmitt <Nancy_Schmitt <@t> pa-ucl.com>; "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Friday, February 15, 2013 6:38 AM
Subject: [Histonet] RE: Type I Water
 
I put tap water in my water bath and haven't had issues.  Alternatively you could use DI water which would be a little cleaner...

Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt
Sent: Friday, February 15, 2013 8:26 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Type I Water

Clearly I sent this too quickly (early) - this is in regards to the water in our tissue float baths - does it HAVE to be Type I?
Thanks
Nancy

Good Morning-
I feel like I am racking up frequent flier miles on Histonet lately:) We are seeing bacteria in our type I.  This requires us to obtain our Type I from another of our lab sites.  Do I have any other options?  Like tap water?
Nancy Schmitt HT, MLT(ASCP)




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