[Histonet] RE: "Vacuole" appearance in lymph Nodes

[Tony Henwood (SCHN)]

Ken,

Are the Microtomists being over enthusiastic with the block "trimming" or "facing"?
This is more prone to happen if the block is cold (ie harder) during trimming.
I would recommend after trimming to full-face that a few slower turns of the microtome wheel at 4-5 microns will clear the holes (if this is indeed the cause of the holes)

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth
Sent: Friday, 15 February 2013 3:32 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] "Vacuole" appearance in lymph Nodes

Hi All,

I have sent this question to respected colleagues individually so pardon my redundancy if you have this email already.

Lately we have had random nodes show up with areas that appear to have multiple vacuoles through-out the specimen.  They have different patterns section to section so I know it is not in the tissue. The staining looks fine and the tissue cuts well so I don't think it is a processing issue. Any ideas on the problem would be welcome. Thanks

Ken


Kenneth G Metzger HTL(ASCP)
Histology Supervisor
ARUP Labs
Salt Lake City, Utah
Phone: (801)583-2787 ext. 3101
Fax: (801) 584-5244
Email: kenneth.metzger <@t> aruplab.com


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