[Histonet] standard protocol

[Tony Henwood (SCHN)]

Hi Kay,

Who was the idiot who sent this to you?

Why bother cutting the sections if they will float off in the xylene, you might as well just send them blank slides.

The companies are willing to spend millions of dollars on the development but couldn't be bothered seeking professional advice before they send out mandatory protocols like this.

Unfortunately I regularly see this and usually send back a letter stating that to follow their protocol we will be charging more for the stupidity. 
I do not tell them what they should be doing unless they are willing to pay for my advice.

You can forward my reply on to them if you so wish.

Here ends my Friday rant!!

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Histopatty
Sent: Friday, 15 February 2013 3:02 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] standard protocol


We are getting instructions from a pharmaceutical company who needs slides, with instructions stating "sections should be floated per standard protocol, xylene dipped from 3 minutes and dried for 10 minutes."  I'm reaching out to those of you who routinly deal with research protocols and wondering is the "standard protocol" the normal float, dri, melt?

Patricia Eneff
for 
Kay Pierce

Please respond to sharon.pierce <@t> hcahealthcare.com if possilbe.  
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