[Histonet] PIN4 staining

[jsjurczak <@t> comcast.net]

Anybody else having problems with PIN 4 staining (Biocare)? We are getting brown background staining, even on the glass itself where there is no tissue. After many years of fantastic results, this problem has occurred recently. Our AMACR is also a lot fainter than it used to be. We've switched out all lots of reagents involved, even bought distilled water. Still no movement towards solving this problem. Any ideas? 
Jeff Jurczak 
Uropartners Laboratory 
Westchester, IL