[Histonet] Processor dehydration cycles

[Parker, Helayne]

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Hello all,

<<<I was wondering what most people use as the first reagent after the formalins on their tissue processor?  We have always used a sequence of 70%, 80%, 95%, and 100% but is anyone using 80% or even 95% to start their dehydration?>>>>

We use start at 95%, but we have two Pen Fix (alcohol fixative) steps between our Formalin and alcohols.  10% NBF, 10% NBF, PenFix, PenFix and then the alcohols starting at 95%.  

Speaking of 10% NBF has anyone ever used un-buffered formalin for routine processing?  I heard of a place using un-buffered formalin and wondered if 1) would that be okay or is it harsh on tissue and 2) would it keep the salt deposits down?  


Helayne Parker, H.T. (ASCP)
Pathology Section Head
Cox Medical Center Branson
P.O. Box 650, Branson, MO 65615
Phone:  417-335-7254
Fax:  417-335-7127
Email:  hparker <@t> skaggs.net
Web:  www.coxhealth.com/branson

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