[Histonet] Counterstain on Ventana Benchmark Ultra

[Goins, Tresa]

We manually stain, but may switch from hematoxylin to Acid Blue 19 to increase contrast with the red chromogen preferred by the pathologists.

Zehntner et al 2008.  Synergistic tissue counterstaining and image segmentation techniques for accurate, quantitative immunohistochemistry.  Journal of Histochemistry and Cytochemistry 56(10):873-880.

Tresa

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Susan Foreman
Sent: Thursday, December 19, 2013 10:38 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Counterstain on Ventana Benchmark Ultra

Merry Christmas to all those out there in Histoland!  

 

How are other labs handling the counterstain for IHC/ICC stained slides on the Ventana Benchmark Ultra?  Has anyone figured out how to counterstain online without purchasing Hematoxylin and Bluing directly from Ventana?  Any
sort of prep kit ?   Our slides are beautiful, just hoping to reduce our
cost/test.

 

Many Thanks,

 

Susan Foreman, HT (ASCP)
(865)661-4734

(865)584-1933

 

 

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