[Histonet] RE: Histonet Digest, Vol 121, Issue 21
Scott A. Ely
sae2001 <@t> med.cornell.edu
Mon Dec 16 15:13:46 CST 2013
re: per slide cost for IHC
I realize there are differences in reagents, including antibody prices. Still, I sometimes am asked to provide the average cost to produce a single IHC slide.
If anyone has an available estimate, especially broken down (glass cost, antibody cost, labor cost...), I would greatly appreciate it.
Thank you.
-------------------------------------------------------------------------------
Scott Ely, MD MPH
Associate Director, Hematopathology Fellowship Program
Section of Hematopathology
Department of Pathology
Weill Medical College of Cornell University
New York Presbyterian Hospital
Room: Starr 715
525 E. 68th Street
New York, NY 10065
PH: 212-746-2442
FAX: 212-746-2009
http://www.cornellphysicians.com/scottely/
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From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of histonet-request <@t> lists.utsouthwestern.edu [histonet-request <@t> lists.utsouthwestern.edu]
Sent: Monday, December 16, 2013 1:07 PM
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Subject: Histonet Digest, Vol 121, Issue 21
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Today's Topics:
1. rabbit cea antibody for mouse (Mehmet Fatih BOZKURT)
2. IHC Lead Tech Position - Dallas (Pat Patterson)
3. Freezer for tissue storage (Wester, Martha)
4. RE: Re: Staining on Alcohol Fixed Smears (Tom McNemar)
5. Charges for recuts on legal cases (Vickroy, Jim)
----------------------------------------------------------------------
Message: 1
Date: Mon, 16 Dec 2013 09:02:54 +0200
From: Mehmet Fatih BOZKURT <fbozkurt <@t> gmail.com>
Subject: [Histonet] rabbit cea antibody for mouse
To: ihcrg <@t> googlegroups.com, Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CA+pSMGTJu1mUUKnNydd45UD76AsXv=_oa_t2NmA+F-2JLq4dDw <@t> mail.gmail.com>
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Hello,
I'm looking for rabbit CEA antibody for using on mouse tissue. Anyone can
help me?
Thanks.
--
Mehmet Fatih BOZKURT, DVM, PhD
Afyon Kocatepe University
Faculty of Veterinary Medicine
Department of Pathology
03030, ANS Campus
Afyonkarahisar-TURKEY
Tel: +902722281312-16173/16237
------------------------------
Message: 2
Date: Mon, 16 Dec 2013 14:41:51 +0000
From: Pat Patterson <Pat.Patterson <@t> propath.com>
Subject: [Histonet] IHC Lead Tech Position - Dallas
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6DCB8B92D0138244B56CE8EACE0D458D1675B38A <@t> Mail.propathlab.com>
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LEAD IMMUNOHISTOCHEMISTRY TECHNICIAN
ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an Lead Immunohistochemistry Technician for its' Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, antibody titer preparation, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording.
The ideal candidate will have a minimum of 5 years Histology experience with paraffin microtomy with a variety of different tissue types, prefer at least 3 years immunohistochemistry, immunofluorescence or in situ hybridization and frozen section experience. Technical Lead/Coordinator experience is required. Working knowledge of IHC theory required, hands on IHC performance desired. If using an automated system we'll easily train you on our manual system. HT (ASCP) or QIHC desired.
The hours for the position are 7:00 p.m. to 3:30 a.m. Monday through Friday.
ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more!
Don't Follow the Leader! Join the Leader!
To apply, please visit www.propath.com
EOE
Accessibility Accommodations
If you require an accommodation to navigate or apply to our careers site, please send your request to accessibility <@t> propath.com.
Pat Patterson, HTL(ASCP)
Supervisor, Immunohistochemistry
ProPath - The Leader in Pathology Services
1355 River Bend Drive
Dallas, TX 75247
pat.patterson <@t> propath.com<mailto:pat.patterson <@t> propath.com>
214-237-1700 x 2027
214-237-1730 fax
To learn more about ProPath, please visit http://www.ProPath.com
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Message: 3
Date: Mon, 16 Dec 2013 14:56:10 +0000
From: "Wester, Martha" <WesterM <@t> MedImmune.com>
Subject: [Histonet] Freezer for tissue storage
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<2FF3ED05D0191D42A22022D82494056D6DD22A42 <@t> MBPRD0002.medimmune.com>
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Dr Cartun-
We've had similar experiences to Hugh's. In the histo group we maybe only have 15 validated freezers (all Revco's), but throughout our company we have hundreds. Over the past few years our Research Operations has moved to purchasing Sanyos because of the turnover rates of the new ultra low Revco's. We have no Sanyo's in our group yet, so I do not have any feedback on their performance. (I've always used uprights, so I'm not much use there either.)
Best of luck!
Martha
Lab Manager
Gaithersburg, MD
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Message: 2
Date: Fri, 13 Dec 2013 11:41:15 -1000
From: Hugh Luk <hlukey <@t> msn.com>
Subject: [Histonet] Freezer for tissue storage
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "Richard.Cartun <@t> hhchealth.org"
<richard.cartun <@t> hhchealth.org>
Message-ID: <BLU168-W478C44F289786B2A012431C3DF0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Dr. Cartun,
Agreed, chest freezers have less frost/door problems/sealing issues and seem to live longer. Stand-ups maximize footprint/space efficiency and allow for easier, horizontal-rack pulling for archiving and inventory. Due to volume and space constraints, the UH cancer center buys stand-ups. We have over a hundred.
My personal caution is for the newer Thermo-Revco freezers, especially the ultra-low Thermo UXF in mostly 24.1 cu. ft.. Perhaps this is just our bad luck but an abnormal number failed (7/9), and three were within the first year. The unfortunate units were purchased over the last four years. Our bio-engineer has asked us to avoid buying Revco (after endorsing Revco/Harris for many years) and buy New Brunswick/Eppendorf or Sanyo ULT freezers, for the immediate future.
We're not endorsing Sanyo or New Brunswick, we're just staying away from a brand that has recently given us trouble. Hopefully, it is different in your area, as Hawaii has logistical issues that may have exacerbated this issue (maybe it was that looong boat trip)?
Just my $0.02. (And $0.02 will not buy anything, anymore.)
Hugh
UHCC path shared resource manager
Honolulu, HI
> ------------------------------
>
> Date: Thu, 12 Dec 2013 18:51:57 +0000
> From: "Cartun, Richard" <Richard.Cartun <@t> hhchealth.org>
> Subject: [Histonet] Freezer for tissue storage
> To: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <9215BD4B0BA1B44D962A71C758B68D2E018A01C8 <@t> HHCEXCHMB05.hhcsystem.org>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I need a recommendation for a -80 degree C. freezer for storing tissue specimens. Do you prefer upright vs. chest? Thanks.
>
>
>
> Richard
>
>
>
> Richard W. Cartun, MS, PhD
>
> Director, Histology & Immunopathology
>
> Director, Biospecimen Collection Programs
>
> Assistant Director, Anatomic Pathology
>
> Hartford Hospital
>
> 80 Seymour Street
>
> Hartford, CT 06102
>
> (860) 545-1596 Office
>
> (860) 545-2204 Fax
>
> richard.cartun <@t> hhchealth.org<mailto:richard.cartun <@t> hhchealth.org>
>
> This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments.
> *****************************************
------------------------------
Message: 3
Date: Fri, 13 Dec 2013 21:57:20 +0000
From: "Truscott, Tom" <ttruscot <@t> vetmed.wsu.edu>
Subject: RE: [Histonet] Freezer for tissue storage
To: Hugh Luk <hlukey <@t> msn.com>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "Richard.Cartun <@t> hhchealth.org"
<richard.cartun <@t> hhchealth.org>
Message-ID:
<9EF5279EBDFE6E4FB6605E8F183A00276AF1503D <@t> CVM77.vetmed.wsu.edu>
Content-Type: text/plain; charset="us-ascii"
We have both upright and chest and both have their pros and cons. We have had trouble with electrical circuits which aren't matched to the load that these freezers can put on them. An electrician told us that it can put an tremendous strain on the freezer motor if the circuit isn't right and two or three freezers start up at the same time- leading to shortened life of the freezer. So- make sure a good electrician is involved in your freezer set-up. Tom T
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hugh Luk
Sent: Friday, December 13, 2013 1:41 PM
To: histonet <@t> lists.utsouthwestern.edu; Richard.Cartun <@t> hhchealth.org
Subject: [Histonet] Freezer for tissue storage
Dr. Cartun,
Agreed, chest freezers have less frost/door problems/sealing issues and seem to live longer. Stand-ups maximize footprint/space efficiency and allow for easier, horizontal-rack pulling for archiving and inventory. Due to volume and space constraints, the UH cancer center buys stand-ups. We have over a hundred.
My personal caution is for the newer Thermo-Revco freezers, especially the ultra-low Thermo UXF in mostly 24.1 cu. ft.. Perhaps this is just our bad luck but an abnormal number failed (7/9), and three were within the first year. The unfortunate units were purchased over the last four years. Our bio-engineer has asked us to avoid buying Revco (after endorsing Revco/Harris for many years) and buy New Brunswick/Eppendorf or Sanyo ULT freezers, for the immediate future.
We're not endorsing Sanyo or New Brunswick, we're just staying away from a brand that has recently given us trouble. Hopefully, it is different in your area, as Hawaii has logistical issues that may have exacerbated this issue (maybe it was that looong boat trip)?
Just my $0.02. (And $0.02 will not buy anything, anymore.)
Hugh
UHCC path shared resource manager
Honolulu, HI
> ------------------------------
>
> Date: Thu, 12 Dec 2013 18:51:57 +0000
> From: "Cartun, Richard" <Richard.Cartun <@t> hhchealth.org>
> Subject: [Histonet] Freezer for tissue storage
> To: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <9215BD4B0BA1B44D962A71C758B68D2E018A01C8 <@t> HHCEXCHMB05.hhcsystem.org>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I need a recommendation for a -80 degree C. freezer for storing tissue specimens. Do you prefer upright vs. chest? Thanks.
>
>
>
> Richard
>
>
>
> Richard W. Cartun, MS, PhD
>
> Director, Histology & Immunopathology
>
> Director, Biospecimen Collection Programs
>
> Assistant Director, Anatomic Pathology
>
> Hartford Hospital
>
> 80 Seymour Street
>
> Hartford, CT 06102
>
> (860) 545-1596 Office
>
> (860) 545-2204 Fax
>
> richard.cartun <@t> hhchealth.org<mailto:richard.cartun <@t> hhchealth.org>
>
> This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments.
> *****************************************
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------------------------------
Message: 4
Date: Sat, 14 Dec 2013 18:25:08 +0200
From: Levi Fried <levi.fried <@t> gmail.com>
Subject: [Histonet] Staining on Alcohol Fixed Smears.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CA+puqxTXL85pEwvR=sqPQA36tMJPm=sx2Kv3GYEbRbu0He3C2g <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi Everyone.
I am looking to stain alcohol fixed smears with a group of antibodies.
The antibodies of particular interest are p63, p53 and ki67.
Along with these antibodies I am looking for positive nuclear and cytoplastic staining antibodies.
If anyone has any experience in staining alcohol fixed slides your information is very appreciated.
All the best.
--
Sincerely,
- Levi Fried
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End of Histonet Digest, Vol 121, Issue 19
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Message: 4
Date: Mon, 16 Dec 2013 11:13:59 -0500
From: Tom McNemar <TMcNemar <@t> lmhealth.org>
Subject: RE: [Histonet] Re: Staining on Alcohol Fixed Smears
To: 'Beth Cox' <bethcoxx <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "levi.fried <@t> gmail.com"
<levi.fried <@t> gmail.com>
Message-ID:
<E9A90E28259D2F4E84308C5E8EA8F7B4016951E94252 <@t> lmhs-exchange>
Content-Type: text/plain; charset="us-ascii"
Since there is no exposure to formalin, we do not do antigen retrieval on alcohol fixed specimens. We use the Ventana Benchmark XT and copy the protocol to a new number and use the "Wet" option rather than paraffin. No retrieval needed.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Beth Cox
Sent: Saturday, December 14, 2013 6:30 PM
To: histonet <@t> lists.utsouthwestern.edu; levi.fried <@t> gmail.com
Subject: [Histonet] Re: Staining on Alcohol Fixed Smears
Levi,
I've worked up most of the more common antibodies on alcohol fixed
smears. There are two things to remember here: 1) your specimens are
alcohol fixed, meaning antigen retrieval needs are different, and 2)
they are smears, meaning the cells weren't 'cut open' during microtomy,
which impeeds some nuclear staining. Those are two different issues to
consider.
Some rules of thumb:
1) most cell membrane antigens will stain nicely with minimal changes
to the protocols you use for FFPE specimens
2) most cytokeratin antigens stain nicely with little change to your
FFPE protocols (if you need to change, start by cutting your antigen
retrieval time in half)
3) most nuclear antigens require significant changes from FFPE
protocols, and some are essentially impossible to stain using standard
antibodies.
When I return to work on Monday, I will forward you my protocol
'adjustments' for the antibodies you requested.
Beth Cox, HTL/SCT(ASCP)QIHC
Vagabond Histotech
On 12/14/2013 12:00 PM, histonet-request <@t> lists.utsouthwestern.edu wrote:
> Message: 4
> Date: Sat, 14 Dec 2013 18:25:08 +0200
> From: Levi Fried<levi.fried <@t> gmail.com>
> Subject: [Histonet] Staining on Alcohol Fixed Smears.
> To:histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> <CA+puqxTXL85pEwvR=sqPQA36tMJPm=sx2Kv3GYEbRbu0He3C2g <@t> mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi Everyone.
>
> I am looking to stain alcohol fixed smears with a group of antibodies.
>
> The antibodies of particular interest are p63, p53 and ki67.
> Along with these antibodies I am looking for positive nuclear and
> cytoplastic staining antibodies.
>
> If anyone has any experience in staining alcohol fixed slides your
> information is very appreciated.
>
> All the best.
>
> -- Sincerely, - Levi Fried
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This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
------------------------------
Message: 5
Date: Mon, 16 Dec 2013 10:16:46 -0600
From: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
Subject: [Histonet] Charges for recuts on legal cases
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Cc: "Bantner, Diane" <Bantner.Diane <@t> mhsil.com>
Message-ID:
<BB0B9F1A8373F14FA2974E8CB24BF9CF7C9C692F <@t> mmc-mail.ad.mhsil.com>
Content-Type: text/plain; charset="us-ascii"
Can anyone share with me what they charge for doing recuts on cases that have been pulled for legal purposes? I am trying to see what others charge before we assign a charge.
I think most people are probably charging by the slide but please let me know if you charge differently.
Thanks
James Vickroy BS, HT(ASCP)
Surgical and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046
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