[Histonet] Freezer for tissue storage

Mon Dec 16 08:56:10 CST 2013

Dr Cartun-
We've had similar experiences to Hugh's. In the histo group we maybe only have 15 validated freezers (all Revco's), but throughout our company we have hundreds.  Over the past few years our Research Operations has moved to purchasing Sanyos because of the turnover rates of the new ultra low Revco's.  We have no Sanyo's in our group yet, so I do not have any feedback on their performance. (I've always used uprights, so I'm not much use there either.)
Best of luck!

Martha
Lab Manager
Gaithersburg, MD
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

Message: 2
Date: Fri, 13 Dec 2013 11:41:15 -1000
From: Hugh Luk <hlukey <@t> msn.com>
Subject: [Histonet] Freezer for tissue storage
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>,    "Richard.Cartun <@t> hhchealth.org"
        <richard.cartun <@t> hhchealth.org>
Message-ID: <BLU168-W478C44F289786B2A012431C3DF0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"

Dr. Cartun,

Agreed, chest freezers have less frost/door problems/sealing issues and seem to live longer. Stand-ups maximize footprint/space efficiency and allow for easier, horizontal-rack pulling for archiving and inventory.  Due to volume and space constraints, the UH cancer center buys stand-ups.  We have over a hundred.

My personal caution is for the newer Thermo-Revco freezers, especially the ultra-low Thermo UXF in mostly 24.1 cu. ft..  Perhaps this is just our bad luck but an abnormal number failed (7/9), and three were within the first year.  The unfortunate units were purchased over the last four years.  Our bio-engineer has asked us to avoid buying Revco (after endorsing Revco/Harris for many years) and buy New Brunswick/Eppendorf or Sanyo ULT freezers, for the immediate future.

We're not endorsing Sanyo or New Brunswick, we're just staying away from a brand that has recently given us trouble.  Hopefully, it is different in your area, as Hawaii has logistical issues that may have exacerbated this issue (maybe it was that looong boat trip)?

Just my $0.02.  (And $0.02 will not buy anything, anymore.)

Hugh
UHCC path shared resource manager
Honolulu, HI

> ------------------------------
>
> Date: Thu, 12 Dec 2013 18:51:57 +0000
> From: "Cartun, Richard" <Richard.Cartun <@t> hhchealth.org>
> Subject: [Histonet] Freezer for tissue storage
> To: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>       <9215BD4B0BA1B44D962A71C758B68D2E018A01C8 <@t> HHCEXCHMB05.hhcsystem.org>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I need a recommendation for a -80 degree C. freezer for storing tissue specimens.  Do you prefer upright vs. chest?  Thanks.
>
>
>
> Richard
>
>
>
> Richard W. Cartun, MS, PhD
>
> Director, Histology & Immunopathology
>
> Director, Biospecimen Collection Programs
>
> Assistant Director, Anatomic Pathology
>
> Hartford Hospital
>
> 80 Seymour Street
>
> Hartford, CT  06102
>
> (860) 545-1596 Office
>
> (860) 545-2204 Fax
>
> richard.cartun <@t> hhchealth.org<mailto:richard.cartun <@t> hhchealth.org>
>
> This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments.
> *****************************************

------------------------------

Message: 3
Date: Fri, 13 Dec 2013 21:57:20 +0000
From: "Truscott, Tom" <ttruscot <@t> vetmed.wsu.edu>
Subject: RE: [Histonet] Freezer for tissue storage
To: Hugh Luk <hlukey <@t> msn.com>, "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>, "Richard.Cartun <@t> hhchealth.org"
        <richard.cartun <@t> hhchealth.org>
Message-ID:
        <9EF5279EBDFE6E4FB6605E8F183A00276AF1503D <@t> CVM77.vetmed.wsu.edu>
Content-Type: text/plain; charset="us-ascii"

We have both upright and chest and both have their pros and cons. We have had trouble with electrical circuits which aren't matched to the load that these freezers can put on them. An electrician told us that it can put an tremendous strain on the freezer motor if the circuit isn't right and two or three freezers start up at the same time- leading to shortened life of the freezer. So- make sure a good electrician is involved in your freezer set-up. Tom T

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hugh Luk
Sent: Friday, December 13, 2013 1:41 PM
To: histonet <@t> lists.utsouthwestern.edu; Richard.Cartun <@t> hhchealth.org
Subject: [Histonet] Freezer for tissue storage

Dr. Cartun,

Agreed, chest freezers have less frost/door problems/sealing issues and seem to live longer. Stand-ups maximize footprint/space efficiency and allow for easier, horizontal-rack pulling for archiving and inventory.  Due to volume and space constraints, the UH cancer center buys stand-ups.  We have over a hundred.

My personal caution is for the newer Thermo-Revco freezers, especially the ultra-low Thermo UXF in mostly 24.1 cu. ft..  Perhaps this is just our bad luck but an abnormal number failed (7/9), and three were within the first year.  The unfortunate units were purchased over the last four years.  Our bio-engineer has asked us to avoid buying Revco (after endorsing Revco/Harris for many years) and buy New Brunswick/Eppendorf or Sanyo ULT freezers, for the immediate future.

We're not endorsing Sanyo or New Brunswick, we're just staying away from a brand that has recently given us trouble.  Hopefully, it is different in your area, as Hawaii has logistical issues that may have exacerbated this issue (maybe it was that looong boat trip)?

Just my $0.02.  (And $0.02 will not buy anything, anymore.)

Hugh
UHCC path shared resource manager
Honolulu, HI

> ------------------------------
>
> Date: Thu, 12 Dec 2013 18:51:57 +0000
> From: "Cartun, Richard" <Richard.Cartun <@t> hhchealth.org>
> Subject: [Histonet] Freezer for tissue storage
> To: Histonet <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>       <9215BD4B0BA1B44D962A71C758B68D2E018A01C8 <@t> HHCEXCHMB05.hhcsystem.org>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I need a recommendation for a -80 degree C. freezer for storing tissue specimens.  Do you prefer upright vs. chest?  Thanks.
>
>
>
> Richard
>
>
>
> Richard W. Cartun, MS, PhD
>
> Director, Histology & Immunopathology
>
> Director, Biospecimen Collection Programs
>
> Assistant Director, Anatomic Pathology
>
> Hartford Hospital
>
> 80 Seymour Street
>
> Hartford, CT  06102
>
> (860) 545-1596 Office
>
> (860) 545-2204 Fax
>
> richard.cartun <@t> hhchealth.org<mailto:richard.cartun <@t> hhchealth.org>
>
> This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments.
> *****************************************

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------------------------------

Message: 4
Date: Sat, 14 Dec 2013 18:25:08 +0200
From: Levi Fried <levi.fried <@t> gmail.com>
Subject: [Histonet] Staining on Alcohol Fixed Smears.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <CA+puqxTXL85pEwvR=sqPQA36tMJPm=sx2Kv3GYEbRbu0He3C2g <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hi Everyone.

I am looking to stain alcohol fixed smears with a group of antibodies.

The antibodies of particular interest are p63, p53 and ki67.
Along with these antibodies I am looking for positive nuclear and cytoplastic staining antibodies.

If anyone has any experience in staining alcohol fixed slides your information is very appreciated.

All the best.

--
Sincerely,
- Levi Fried

------------------------------

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End of Histonet Digest, Vol 121, Issue 19
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