[Histonet] M2D2 and SMAD

[Hoekert, W.E.J.]

Hi,
 
SMAD4 is very difficult on the Ventana platform. The only clone that would work on the Ventana to my knowledge(clone EP618Y, immunologic) has recently 'collapsed' (i hope that's the right word?) It means that it is not available anymore. Maybe there is some vendor that has a few vials left, but my supplier does not. If anybody knows of some other clone for the Ventana, I am very interested.
 
MDM2: our sarcoma pathologist says that the MDM2 immuno is not reliable. Some liposarcomas will be negative. It would be better to do FISH, which is also very difficult because the fatty tissue often rinses off the slides. 
 
 
Willem
 

________________________________

Van: histonet-bounces <@t> lists.utsouthwestern.edu namens Sue Hunter
Verzonden: do 5-12-2013 16:50
Aan: Histonet <@t> lists.utsouthwestern.edu
Onderwerp: [Histonet] M2D2 and SMAD



Good morning
Does anyone have suggestions for antibodies for M2D2 and SMAD?  We have not been able to get these antibodies  to work for us on the Ventana Ultras.
If you do have suggestions, protocols and control tissue suggestions would also be appreciated.
Thanks
Sue

Sue Hunter, Supervisor
Advanced Diagnostics
Beaumont Health System
Royal Oak MI
248-898-5146
shunter <@t> beaumont.edu<mailto:shunter <@t> beaumont.edu>


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



Disclaimer: 

Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. 
In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend.