[Histonet] Rush bx schedule?

[Cristi Rigazio]

We do strictly GI biopsies, but if we have a stat case, we have a processing schedule as follows: five minutes in each station of one 70%, two 95%, two 100% and three xylenes, then 10 minutes in each of three paraffins.  We have the ASP300 and this has been very successful.  I have heard from others they do as little as 2 minutes in each, With a bit longer in the paraffin.  Generally our STAT cases are in formalin overnight so we skip that step altogether.
Happy Holidays!
Cristi

Sent from my iPhone

On Dec 4, 2013, at 9:31 AM, "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org> wrote:

> For tissue, what step most contributes to subsequent swelling of tissue when the block is soaked? I'm thinking the 100% ETOH, xylene clearing and paraffin infiltration all contribute, but at what percentage?
> 
> Here is the current schedule, which apparently was meant to mimic a microwave schedule that was used before the MW quit working. The tissue - kidney and liver bx for the most part, up to 10 per day, is coming out soft and swells a bit when soaked. So sectioning is difficult.
> 
> We need to have a rapid schedule but need good sections. Obviously we are pushing the limits on this so need a bit of leeway for variation in tissue
> 
> So, I'm considering which of these steps is the most critical to lengthen.
> This is on a VIP5 but we can also use a peloris for this processing
> 
> Formalin 2 min, 50degC (this is not necessary at all, IMHO)
> 80% ETOH 5 min, 50degC
> 95%ETOH 5 min, 50degC
> 100% ETOH 8 min, 50degC
> Xylene, 9 min 50degC
> Paraffin, 4 min,
> Paraffin, 5 min
> 
> Thanks for your insight!
> 
> 
> 
> Tim Morken
> Supervisor, Electron Microscopy and Neuromuscular Special Studies
> UC San Francisco Medical Center
> Box 1656
> 505 Parnassus Ave
> San Francisco, CA 94143
> USA
> 
> 415.353.1266  (office)
> tim.morken <@t> ucsfmedctr.org<mailto:tim.morken <@t> ucsfmedctr.org>
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet