[Histonet] (no subject)

[Amos Brooks]

Hi,
    There are a few ways of doing this. Perhaps the easiest  is scraping the cells off while it is still in the media. Transfer it to a centrifuge tube and spin it down. Pour off the supernatant add formalism vortex it and centrifuge it again and pour off the supernatant again and add Histogel or agar. Then process as usual. This is about as close as you are going to get to treating it like normal tissue.

Amos


Message: 4 Date: Mon, 2 Dec 2013 17:06:24 +0000 From: Mike Tighe <mtighe <@t> trudeauinstitute.org> Subject: [Histonet] Embedding cells in Paraffin To: "histonet <@t> lists.utsouthwestern.edu (histonet <@t> lists.utsouthwestern.edu)" <histonet <@t> lists.utsouthwestern.edu> Message-ID: <46d5589df0864870848089e6780fb432 <@t> BY2PR07MB469.namprd07.prod.outlook.com>

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Has anyone tried to embed cells grown in tissue culture? I am trying to put some tissue culture cells through same stress as tissue would go through. Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and then fix for extended time with NBF but that doesn't quite seem fair.

Thanks for any ideas!