[Histonet] Isopropyl Alcohol in the histology lab

[Rene J Buesa]

The tissue processor can be cleaned with a strong glassware laboratory liquid soap at 5% in either hot water or hot IPA. The "hot" is the temperature provided by the tissue processor during the cleaning cycle.
After you dry the stained sections in an oven at 60ºC for 5 minutes, you can place them directly in the coverslipper and they will be covered without any problems.
René J.


________________________________
From: Brendal Finlay <brendal.finlay <@t> medicalcenterclinic.com>
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Tuesday, August 20, 2013 6:36 AM
Subject: RE: [Histonet] Isopropyl Alcohol in the histology lab



René,


I'm very interested in your xylen free methods. I have a few questions
for you, if you don't mine answering them.  What is used on the clean
cycle of your processor to remove paraffin waste?  Our lab has a
glass coverslipper.  How would this work with coverslipping the
slides manually or with our coverslipper?  Thank you for all the
information you offer to us at Histonet!

Brendal Finlay, HT (ASCP)

-----Original message-----
From: Teri Johnson TJohnson <@t> gnf.org
Date: Tue, 13 Aug 2013 13:59:39 -0500
To: "Rathborne, Toni" trathborne <@t> somerset-healthcare.com, 'Rene J
Buesa'rjbuesa <@t> yahoo.com, "'gu.lang <@t> gmx.at'" gu.lang <@t> gmx.at
Subject: RE: [Histonet] Isopropyl Alcohol in the histology lab


Teri:
The automatic coverslipper will wok on oven dried stained sections. I
used them on a Sakura film coverslipper and my lab was in Miami Beach,
and you do not more humid than that!
Xylene isthe one weakening the immunoreactivity the most but I have
tested the IPA and the weakening does not exist although there are so
many antibodies that some may weaken the issue is: will the weakening
affect the diagnosis or just will produce a weaker reaction? That you
would have to test further.
But the bottom line is tha xylene should be eliminated.
René  J.

Hi Rene,

Have they changed the film coverslipper technology? When I used it
years ago, you needed xylene to drop on to the slides to affix the
film to them.
The film coverslipper is not an option for us, we need the optical
resolution that regular coverslips give for slide scanning.

~Teri

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