[Histonet] Positive tissue controls for MSI antibody panel

[Sebree Linda A]

Question everyone:

What are you all using for your positive tissue control when running the MSI antibody panels, i.e. MLH-1, MSH-2, MSH-6, PMS-2?  We're contemplating using normal colon that all four antibodies should stain but do we also need to show that colon having the mutation does not stain for each antibody as well to show there are no false positive results?

The former would be the easy way out but I'm willing to construct a multi-tissue block containing normal colon and colon with the mutation(s).

Thanks for all your responses,

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174