[Histonet] edge artefact on core needle biopsy

[Tony Henwood (SCHN)]

Hi Gudrun,

I hope you are well.

Another cause of anomalous staining is drying of the biopsy prior to fixative immersion.
This can result in non-specific immunohistochemical staining as well as sub-optimal histochemical staining.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Saturday, 17 August 2013 7:19 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] edge artefact on core needle biopsy

Hi all!

We had a discussion on the cause of IHC positivity/false-positivity on the edges of core needle biopsies. (eg. breast, ER)

One said, it is due to overfixation with formaldehyde-concentrations above 5%. (=false pos.)

My opinion is, that's it's rather an effect of underfixation in the center in those cases. (= real pos.)

 

In my lab, we rarely see this margin-effect and the biopsies are fixed for 6 hours at least.

I searched in Pubmed for publications on this assay, but without success.

Can anyone help me out with any literature? Or personal experience?

 

Thanks in advance

 

Gudrun Lang

Ltd. BMA Histolab Linz, Austria

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