[Histonet] RE: Biocare Decloaker Nx Gen

[Ian R Bernard]

We recently bought this equipment and it works well. We are happy with it.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Thursday, March 28, 2013 1:52 PM
To: Swartwood, Steven J; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Biocare Decloaker Nx Gen

We have used the Decloaking Chamber for several years now and have had excellent results. The nicest thing is that there is consistency and reliability.  Each run will be the same as the one previous and having the ability to use the USB stick for documentation of time, pressure and temperature makes record keeping really easy.  We have had no complaints.  The only con I can come up with is that it comes in a big box that is hard to store if you have to send it back for some reason.

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lblazek <@t> digestivespecialists.com




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Swartwood, Steven J
Sent: Thursday, March 28, 2013 1:40 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Biocare Decloaker Nx Gen

Has anyone ever used the Biocare Decloaking Chamber Nx Gen for epitope unmasking (heat retrieval)? Also does anyone have any personal pros/cons with this instrument? We are thinking about getting one because of inconsistencies with our current method using a vegetable steamer. We use many different tissue types both animal and human. Also we use many different antibodies. Currently with the vegetable steamer we've used Citrate, EDTA, and Tris-EDTA combinations. We've used times from 30-60 minutes, and even tried 30 minutes x 2 or 40 minutes x 2, but we still see some inconsistent staining. To make sure it wasn't due to fixation vimentin staining was performed and shows even staining. It's just certain antibodies that are giving us trouble even from multiple different companies. Any advice would be greatly appreciated and very beneficial to our work pertaining to the instrument.

Thanks again everyone have a great end of the week,

Steven Swartwood HT(ASCP)
steven.swartwood <@t> cshs.org

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