[Histonet] IHC after EDTA decalcifying

[pruegg <@t> ihctech.net]

Colleen,

The antigen expression you are getting in the human tonsil may not be
expressed in the mouse femur, plus u have to make sure the ab u are using
cross reacts with mouse tissue, if it is anti human it may or may not react
in mouse, the ab data sheet should indicate species reactivity.  The edta
should not be the problem here.

Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm <@t> hotmail.com 
rueggihcconsultingpr <@t> outlook.com



 

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Colleen
Forster
Sent: Thursday, August 15, 2013 5:59 PM
To: Histonet
Subject: [Histonet] IHC after EDTA decalcifying

Hello fellow histonetters,

Can someone who has worked with decalcifyied bone tell me if EDTA interferes
with IHC staining? I was under the impression it did not but cannot get
staining in mouse femurs that I have decaled in EDTA. I have used both the
steamer and the decloaker for retrieval and in the human tonsil the stain is
great.

Any suggestions......

Thanks in advance!

Colleen Forster
U of MN

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