[Histonet] Cryostat cleaning

[Morken, Timothy]

Ann, 

There are many tuberculocidal cleaners. Just search for it. If you are in a hospital the central supply will most likely have some in stock.  
We use PD-128 by Spartan Chemical  

http://www.spartanchemical.com/products/product/101604


Here are some references for cryostat cleaning:

Cryostat decontamination references:

College of American Pathologists, Commentary on certification questionnaire:
1)	Decontaminate regularly with 70% alcohol.
2)	Defrost, remove trimmings and decontaminate with a tuberculocidal disinfectant, preferably weekly for instruments used daily
3)	Reference: National Committee for Clinical Laboratory Standards (NCCLS), Protection of Laboratory Workers from Instrument Biohazards and Infectious Disease Transmitted by Blood, body Fluids, and Tissue; Approved Guideline M29-A. Wayne, PA, NCCLS, 1997

NCCLS site: http://www.nccls.org/


Decontamination for HIV
Frozen Section Technique for Tissues Infected by the AIDS Virus, Swisher, B.L., Ewing, E.P., J histotechnol, V9, No.1, p.29 (March 1986)
Recommends 95% ETOH (other publications referenced here indicate 95% alcohol is effective against HIV).

Department of Agriculture for Northern Ireland, Safe Working and the Prevention of Infection in Clinical Laboratories, Health Services Advisory Committee. 
Recommends formalin fuming for 24-48 hours at room temperature. Followed by ammonia for one hour (presumably as a neutralizer).

Laboratory Histopathology: A Complete Reference, Woods and Ellis, Churchill Livingstone, 1994.
Recommends defrosting , washing with 70% alcohol, complete cleaning. (no references given).

For those who like to read a lot:
APIC Guideline for Selection and Use of Disinfectants, Association for Professionals in Infection Control (APIC), American Journal of Infection Control, Vol 24(4), August 1996, 313-342. A good review of all disinfectants in use and the pros and cons of each.


Tim Morken
UCSF Medical Center

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ann Specian
Sent: Friday, August 16, 2013 8:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cryostat cleaning


ANP.12087 states that the cryostat needs to be defrosted and wiped clean with a tuberculocidal.

Can someone tell me what they use that meets the CAP criteria listed above.

thankis, 
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