[Histonet] Re: One step trichrome kits

[joelle weaver]

I use muscle for muscle bx, and liver exclusively for other trichrome. It is not ordered as frequently, definitely. I find it sad that you felt you had to mention that histotechs should look at each slide...but of course, why would you NOT check each under the microscope?  Sorry, I know that wasn't the point of this string- but to me this is basic quality control.




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> Date: Thu, 15 Aug 2013 11:32:40 -0400
> From: rsrichmond <@t> gmail.com
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: One step trichrome kits
> 
> About the only use the working surgical pathologist has for trichrome
> stains these days is to evaluate fibrosis in liver biopsy specimens, nearly
> always for hepatitis C.
> 
> For this purpose I need a blue trichrome stain that will stain fine fibrous
> tissue brightly and intensely. I've seen one-step kits that did this, and
> also seen a great many that didn't. I think the kits probably go bad with
> age.
> 
> Distasteful though the task may be, the histotechnologist really must check
> the liver biopsy slides each time the stain is run.
> 
> The control should be surgical or autopsy liver, normal, or better with
> slight fibrosis. Not uterus or bowel.
> 
> One-step kits for muscle biopsy frozen sections are a different issue. I
> used to brew the Gomori-Engel-Cunningham brew myself - it lasted about a
> week - but haven't done them since 1970, and can't comment on kits. I also
> haven't seen kits for general-oversight trichromes such as the
> hematoxylin-phloxine-safffron (HPS) technique, if there are any labs still
> doing it.
> 
> Bob Richmond
> Samurai Pathologist
> Maryville, Tennessee
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