[Histonet] Isopropyl Alcohol in the histology lab

[Tony Auge]

I recently switched to isopropanol in my lab. One thing I did first, that
you may want to consider, was to check with the manufacturers of my
processors and recyclers that isopropanaol would be compatible.

1. Can't help you with this one
2. I did have to adjust the staining times for eosin and the dehydration
after.
3. I have not had any IHC staining issues since switching.
4. I still use reagent alcohol on my Artisan special stainer. DAKO said it
would not be compatible.

Tony Auge HTL (ASCP) QIHC
Cell: (651) 373-4768
Email: tony.auge <@t> gmail.com

On Mon, Aug 12, 2013 at 2:18 PM, Teri Johnson <TJohnson <@t> gnf.org> wrote:

> Dear colleagues,
>
> I am entertaining the notion of switching over to isopropyl alcohol (IPA)
> for our tissue processing and have a few questions. I know the xylene-free
> processing and microwave processing protocols use this universally, but at
> this time I am only interested in getting rid of the
> ethanol/methanol/isopropyl alcohol blend.
>
> 1 - Are there any known issues with holding tissues in 70% IPA rather than
> 70% ethanol/reagent alcohol?
> 2 - Is there any impact on the stain line if you use it for H&Es and
> hydration/dehydration of histochemically stained slides?
> 3 - Have any of you who use IPA found any impact on immunoreactivity when
> compared to using other dehydrants?
> 4 - Is there anything else I have overlooked?
>
> We have pure ethanol available to us if needed for particular stain
> applications.
>
> Best wishes, and thanks in advance.
>
> Teri Johnson
> Manager, Histology
> GNF - San Diego, CA
> 858-332-4752
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



--