[Histonet] Cryostat Cleaning

[Morken, Timothy]

Ann,

Cryostat decontamination references:

 

College of American Pathologists, Commentary on certification questionaire:

1)     Decontaminate regularly with 70% alcohol.

2)     Defrost, remove trimmings and decontaminate with a tuberculocidal disinfectant, preferably weekly for instruments used daily

3)     Reference: National Committee for Clinical Laboratory Standards (NCCLS), Protection of Laboratory Workers from Instrument Biohazards and Infectious Disease Transmitted by Blood, body Fluids, and Tissue; Approved Guideline M29-A. Wayne, PA, NCCLS, 1997

http://www.cap.org/HTML/checklist_html/cklst08p.html

 

NCCLS site: http://www.nccls.org/

 

 

Decontamination for HIV

Frozen Section Technique for Tissues Infected by the AIDS Virus, Swisher, B.L., Ewing, E.P., J histotechnol, V9, No.1, p.29 (March 1986)

Recommends 95% ETOH (other publications referenced here indicate 95% alcohol is effective against HIV).

 

Department of Agriculture for Northern Ireland, Safe Working and the Prevention of Infection in Clinical Laboratories, Health Services Advisory Committee. 

Recommends formalin fuming for 24-48 hours at room temperature. Followed by ammonia for one hour (presumably as a neutralizer).

 

Laboratory Histopathology: A Complete Reference, Woods and Ellis, Churchill Livingstone, 1994.

Recommends defrosting , washing with 70% alcohol, complete cleaning. (no references given).

 

For those who like to read a lot:

APIC Guideline for Selection and Use of Disinfectants, Association for Professionals in Infection Control (APIC), American Journal of Infection Control, Vol 24(4), August 1996, 313-342. A good review of all disinfectants in use and the pros and cons of each.


Tim Morken
Supervisor, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ann Specian
Sent: Tuesday, August 06, 2013 12:09 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cryostat Cleaning


Can someone tell me the CAP approved chemical that can be used to clean the cryostat?
thanks, Ann
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