[Histonet] antibody diluent

Eva Permaul eca9 <@t> georgetown.edu
Tue Apr 9 14:37:31 CDT 2013


We usually just use 1xTris buffered saline with 0.05% Tween.

Eva


On Tue, Apr 9, 2013 at 3:34 PM, Geoff <mcauliff <@t> umdnj.edu> wrote:

> You could make your own. It is usually just a buffer with some normal
> serum and Triton or Tween added.
>
> Geoff
>
>
> On 4/9/2013 2:14 PM, Perry, Margaret wrote:
>
>> I am looking for a less expensive antibody diluent and would like some
>> opinions on which companies are best .  Are there differences between
>> diluents when it comes to background staining?
>>
>> Margaret Perry HT(ASCP)
>> Veterinary & Biomedical Sciences Department
>> North Campus Drive Box 2175
>> South Dakota State University
>> Brookings SD 57007
>> 605-688-5638
>>
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>
>
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> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
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