[Histonet] Nail treatment and bone marrow decalcification info needed

[Laurie Colbert]

We purchase a solution called Nail Prep from Stat Lab for our nails.  It works wonders!  You place the nails in the solution for about 1 hour, rinse, and process as usual.

Laurie Colbert, HT (ASCP)

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of carmen loiselle
Sent: Wednesday, April 03, 2013 1:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Nail treatment and bone marrow decalcification info needed

Hello fellow histonetters ,

I need your expertise regarding the
treatment of nails.  We currently immerse the nails specimen in a solution of tween & formalin up to 2 weeks .  We then process and section them.  Is there a more rapid and efficient way to do it ???

 
Also,
 we recently change our procedure for the fixation of the bone marrow specimen.  Up to last week we were using Zenker's solution .  Starting in april we are now using the formalin fixation for 24 hours before 
decalcification.   What are the most efficient and rapid way of doing 
the decalcification without altering the IHC markers  currently use for these type of specimen ???
At the present time we are using 10%
formic acid for 2 hours, rinse then process.  What about RDO agent (which I believe is HCL based)  which is faster ????

Thank you in advance for all your responses

Carmen 		 	   		  _______________________________________________
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