[Histonet] Re:Rabbit antibody on rabbit tissues

[Galina Deyneko]

Dear Colleagues
 My current problem is: I should detect the myeloperoxidase  (MPO) antigen and its derivate nitrotyrosine and chlorotyrosine in the rabbit tissues (aorta) . Most of the antibodies what I found and claimed to work on Rabbit are rabbit polyclonal. Do you know  antibodies for those antigens from any other species or biotinylated antibodies, or you can suggest good secondary antibodies and detection systems that might work on rabbit tissues with rabbit primary, on the analogue of mouse-on-mouse detection system.
I already tested goat and mouse anti-human MPO on rabbit aorta and lung/liver as a positive control, but  besides stained macrophages and endothelial cells, i got stained smooth muscles in the media, and I am not sure that the staining in macrophages is specific. Maybe yes, since negative controls with mouse and goat IgG are absolutely clean.
Can you share you advices, opinion, experience.
 
Thank you in advance


Galina Deyneko
Novartis, Cambridge, MA
 
617-871-7613 w

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