[Histonet] Re: Cooling paraffin blocks with ice VS. Freezing Spray

Hobbs, Carl carl.hobbs <@t> kcl.ac.uk
Sun Sep 30 13:04:01 CDT 2012

I don't like ice-trays.
Well, I tried them and.....they swelled me blocks!
Stick them blocks in the fridge, instead!

Sure, cooling them blocks is damn fine to get very thin kidney sections...

The wonderful Chief Tech of Gt Ormond St kids Hospital.... Where are you???
You know all this stuff abart getting THIN kidney sections from Pwax blocks.

Try "hughing" on the block, before cutting?
Like a kid's breath when they are sleeping.....
It's warm and, momentarily , expands them wax crystals?
NB: Cooling them will have the same effect......weirdly



Caveat: each Lab MUST test out so many variables so as to appear Anal..
Fine...if you get damn fine results.
However, share them results.

Carl Hobbs
Histology Manager
Wolfson CARD
School of Biomedical Sciences
Kings College London
Guys Campus
Tel: 020 78486813
Fax: 020 78486816
020 78486813

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