[Histonet] freeze spray vs ic for microtomy There is a petient on
the other side of the coverslip
madary <@t> verizon.net
madary <@t> verizon.net
Sun Sep 30 09:41:25 CDT 2012
&nb method, and When Lee Luna wa are as varied as technici putting out an acceptable number and your boss needs to accept your method as on Regarding freeze sprays, never been a big fan, I sti believe there are safe for the ozone. Respect your mgrs differenc and prove to her you can be just as effective. I have been on both
side several times SOmetimes you have is a person on the other s
Nick(Rocky) Madary, HT/HTL(ASCP)QIHC
< On 09/29/12, [DEL: histonet-request <@t> lists.utsouthwestern.edu :DEL]
wrote:
&nbs [1]histonet <@t> lists.u To subscribe or unsubscribe via the World Wide [2]http://lists.utsouthwestern.ed or, via email, send a message with subje [3]histonet-request <@t> lists.utsout You can reach the person managing the list at
[4]histonet-owner <@t> lists.utsouthwestern.edu
When r than "Re: Today's Topics:
1. Re: Pa 2. Caspase 8 for Mouse (Elizabeth Ca 3. Stainer for sale (Adrienne Anderson)
4. Re: Histonet Digest 5. Cooling paraffin blocks with ice 6. Re: Cooling paraffin blocks with ice 7. Cooling paraffin blocks with ice VS. Fre (Contact HistoCare)
8. AW: [Histonet] Cooling paraffin bl Spray (Gudrun Lang)
9. RE: Cooling paraffi (joelle weaver)
10. Re: Cooling (Jackie O'Connor)
11. Re: (Rene J Buesa)
< -------------------------------------------------------------------- --
Message: 1
Date: Fri, 28 Sep 2012 13:05:22 -0400 (EDT)
From Subject: Re: [Histonet] Para To: [6]deshsmith1 <@t> gmail.com, histonet <@t> li Message-ID:
<8CF6BB[7]294563E1E-1274-49EA7 <@t> Webmail-m125.sysops.aol.com>
Content-T It has been my experience long (like over a weekend) become embedding over 300 blocks, those blocks may rem station for up to 6 hours - but I personally strongly sticking to your SOP for processing. Besides, if your SOP says pa raffin for 3 hours, leaving them longer is a violation of your SOP.
Jack -----Original Message-----
From: Demetria Ross < To: histonet <histonet <@t> li Sent: Thu, Sep 27, 2012 5:21 pm
Subjec I'm curious to know how lo paraffin
efore it becomes a problem min-2
ours before I take it off _____________________________ istonet mailing list
[9]istonet <@t> lists.uts ttp://lists.utsouthwestern.edu/mailman/listinfo/hist ------------------------------
Message: 2
Dat From: Elizabeth Cameron <[10]El Subject: [Histonet] Caspase 8 for Mouse< "[11]histonet <@t> lists.utsouthwestern.edu"
<[12]histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BE1B Content-Type: text/plain; charset="us-ascii"
Anyone tissue?
Thanks!-Liz
The information in this email, including attachments, may confidential and is intended solely for the addressee(s). If you
believ by return em ------------------------------
M Date: Fri, 28 Sep 2012 15:26:04 -0400
From: Adrienne Anders Subject: [Histonet] Stainer for s To: [15]histonet <@t> lists.utsouthwestern.edu
Message-I Content-Type: text/plain; charset=us-ascii
Hello all,
We h DS50. If int Adrienne
------ Message: 4
Date: Fri, 28 Sep 2012 14:24: From: Galina Deyneko <[17]galinadeyneko <@t> yahoo.com& Subject: [Histonet] Re: Histonet Digest, Vol 106, Issue 35
To: [18]histonet <@t> lists.utsouthwestern.edu
Message-ID:
<134 .bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Dear Colleagues
I would like to ask again about cell block preparat found number of answer on the histonet site and sorry that again.
The cells what I prepare look distorted. Short protocol: 30 of fixation in 10 %NBF, centrifuge 1000 rmr, wash in PBS, ce ntrifuge, re-suspend in histogel and processed in Thermo Shandon with
follo changes, Wax 3 protocol - 30 minutes I also would like to try to do OCT embedding to avoid anparaffinin embedding. Should I fix the cells before OCT embedd or not? Can I re-suspend directly in OCT or still need to embed in
Hist Please could you sha Thank you and good weekend.
Galina Deyneko
Novartis, Cambridge, MA
617-871-761
------------------------------
Mess Date: Fri, 28 Sep 2012 22:39:31 -0400
From: Jenny Vega <[20] Subject: [Histonet] Cooling paraffin bloc Spray
To: [21]histonet <@t> lists.utsou Message-ID:
<CADyQr2DtWq9Fb6f7ByrK65aVg_8bCP[22]hEYGhYWvDWE0L8mVVVSA <@t> mail.gmail.co
m>
Content-Type: te I want to know what is your preferred blocks in
the microtome everyday. At work I because we have different ways of d doesn't
like to use the technique where an
ice tray and then make a secti histotech
school. Instead she just t by one
using the same blade un only
freezing spray.
Sh to heris a waste of time and that is why I have to use her technique
but
unf to go
b wrin minutes. I
them
with ice I don't need to use freeze spray that much. Her technique
worksbut is more successful when the blocks are well processed. I have
diff trying
to times
blades< section. I prefer tw separate blades, one to trim and the other one to section. I feel
they sharp for more time.
She discourages the use of ice but the running out
of freezing spray for the frozen sec make
sense. It is obvious that if she en blocks then
we will be using less freezing s Another reason she discourages the use of ice is that some blo are not
meant to be chilled which is pretty understandable. I cannot small
biopsies such as gastric and skin and bone because they can hard
and tear off from the block so I avoid that but I prefer to breast and
colon biopsies on ice because these are fatty tissue th tedious to
cut even when relying only on freezing spray.
I want to know if it's completely acceptable for me to prefer the trim,
cool on ice and section technique and if you feel is a waste of t comparing it with other ways of cutting such as the one I mentioned. Thanks.
------------------------------
Me Date: Sat, 29 Sep 2012 00:04:29 -0300
From: "C.D.G." <[23]latecor <@t> montevideo.com.uy>
Subject: Re: [Histonet] Cooling par Freezing
Spray
To: histotech411 <@t> gmail.co Message-ID: & lt;2012092[25]90004290984.0018B2E0 <@t> smtp.montevideo.com. Content-Type: text/plain; charset="ISO-8859-1"
Freez much could like partial "holes" on the section, so and not always, as you stated, some
pieces cut The use of ice is possible,but I water over the blade holder unit, leads t slow but progressive corrosion of some of the components that are i ndispensable for an accurately sectioning work.
Try to begin using the s materials and you'll soon be feeling c with this method.
My best regards ,
Carlos.-
******* On 28/09/2012 at 10:39 p.m. Jenny V >I want to know what is your preferred method for cutt blocks
>in
>the microtome everyday. At work I am h supervisor
>because we have different ways of do doesn't
>like to use the technique wh on an
>ice tray and then make histotech
>school. Instead by one
>using th only
>freezi >
>She doesn't like to cool the blocks on an ice tray to her
>is a waste of time and that is why I have >unfortunately some blocks are extremely dif to go
>back to my preferred technique. I fee without
>wrinkles when I chill and soak the b minutes. I
>sometimes use freeze spray w them
>with ice I don't need t works
>but is more succes >difficulty getting c trying
>to get the per times
>is tedio blades
>g prefer two
>separate blades, one to trim and the other one to section. I feel
they >sharp for more time.
>
>She discourages the use of running out
>of freezing spray fo make
>sense. It is ob blocks then
>we will >
>Another reason she discourages are not
>meant to be chilled whic small
>biopsies such as gas hard
>and tear off fr breast and
>colon b tedious to
> >
>
>
& the trim,< waste of time >comparing it with other ways of cutting such as the one I mentioned .
>
>
>
>Thanks.
>___________________________ >Histonet mailing list
>
>[26]http://lists.utsouthwestern.edu/mailman/listinfo/hist ------------------------------
M Date: Fri, 28 Sep 2012 23:30:10 -0500
From: Contact HistoCa Subject: [Histonet] Cooling para Spray
To: "histonet <@t> l <[28]histonet <@t> lists.utsouthwes Message-ID: <BA775FF7-0667-4B[29]B0-8AC7- Content-Type: text/plain; charset=u Hi, I have a feeling that the supervisor's motivation for di scouraging your personal technique is financial and not procedural. I
can't especially insistence on on like skin or GI.
When you have only one way to skin your cat, figuratively speaking,
somet excess. For examp through a lot more blades. sharp, high quality blade to cut an ice tray to keep your blocks cold hel farther.
Remember, patient care should never use a fresh blade to get the best section, and reasonable pathologist who wouldn't side with A skilled histotech who is proficient in cutting can use ice tr and not waste any time. As a point of reference, I can face(or trim)
AN That's VERY It is more likely that one would have to wrestle with a w temperature block longer with using only spray to get the best section.
You are an artist and there are many techniques to get the results in creating your masterpiece. I would certainly be receptiv to learning different techniques from your supervisor to ADD to your
repe within rea in ways to be mo familiar with.
[30]www.HistoCare.com
--------------------- Message: 8
Date: Sat, 29 Sep 2012 10:32:35 +0200
Fro Subject: AW: [Histonet] Cooling p Freezing
Spray
To: "'Jenny Vega'" <[32] Cc: [33]histonet <@t> lists.utsouthw Message-ID: <008d01cd9e1c$fb090e40$f11b2ac0$@gmx.at> Content-Type: text/plain; charset="iso-8859-1"
Cooling on ice trimming and
consecutiv We use cooling devices a snow-surface, that gives the blo Especially
blocks, that have to be recu to cut.
I think freezing spr evan
temperature throughout is
better to cut.
I a puttingaway and then cutting again. - but- I work with a sliding
microtome andtrimming goes really fast.
Gudrun Lang
-----Ursprü Von: [34]histonet-bounces <@t> lists [[35]mailto:histonet-bounces <@t> li von Jenny Vega
Gesendet: Samstag, An: [36]histonet <@t> lists.utsouthwester Betreff: [Histonet] Cooling paraffin blocks with ice VS. Freez Spray
I want to know what is your preferred method for cutting p blocks in
the microtome everyday. At work I am having issues wit supervisor
because we have different ways of doing things like for doesn't
like to use the technique where you first trim the t on an ice
tray and then make a section. That is how I lea histotech
school. Instead she just trims and cuts the blo by one
using the same blade until it wears out and only
freezing spray.
She doesn't like to coo to her
is a waste of time unfortunately some block to go
back to my preferred t wrinkles when I chill and minutes. I
sometimes use freeze them
with ice I don't nee works but
is more success difficulty
getting comple get the
perfect sect tedious
using this worn
down quic separate
blades, sharp for
mo She discourages the use of ice but then complains that we a running out of
freezing spray for the frozen sections too quickly whi make sense.
It is obvious that if she encourages to use ice t we will
be using less freezing spray.
Another are not
meant small
biopsi hard and
t and
c tedious to< I want to k trim, cool
on ice comparing it
w Thank _______________________________________________
Histonet mailing l [37]Histonet <@t> lists.utsouthwestern.edu
[38]http://lists.utsouthwestern.edu/mailman/listinfo/histonet< ------------------------------
Message: 9
From: joelle weaver <[39]joe Subject: RE: [Histonet] Cooling paraffin b Freezing
Spray
To: <[40]histotech411 <@t> gmail.com>
Cc: [41]histonet <@t> lists.utsouthwestern.edu
Messag C Jenny My experie or at the very least a also was taught this method in training at four quite large institutions, variation of an ice cooling method in every instanc working in clinical and research settings. There are always variations in technique from lab to lab, but freezing spray has been
genera can introduce a to see the effect on even under the microscope.) spray personally for regular paraffin doing frozen sections on occasion, but then it difficult specimens such as fatty breast or soft/fat that need very cold temperatures. I sometimes use it to coo only the backside of paraffin blocks during embedding when I am being
about the prefer my blocks qu currently work , is that temperature after removal from the embedd I can more efficiently get good sections when t is maintained and uniform though the block, rather than warmed room temp. block. Overall, I would expect constant and application of the freezing spray would be more of a problem than a nything involving ice, which would "flash freeze" mostly the surface,
and n Of course, I the ice for so lon you are sitting at your mic diligently, and not leaving faced pecimens just sit t sure how this would be an issue. In general I think the combi of ice and water benefits most specimens ( especially GI and Liver c ores, bloody stuff, and other types-that can sometimes be brittle and
delic that transf reduces brittleness of t feel that I would be unable to g hard/dense tissues such as uterus body, cerv others without using ice. The only exception for m which can cut better warm. I am sure you must be frustrat this is the clear direction of your supervisor, and they are not receptive to making any changes or allowing you to use your preferred
tech not sure that other than comply with their polici are not open to new ideas and techniques experience and those feelings quite often over the years, try to stay postive, do the best you can.
Joelle > Date: Fri, 28 Sep 2012 22:39:31 -0400< [43]histotech411 <@t> gmail.com
> To: [44]histo > Subject: [Histonet] Cooling paraff Spray
>
> I want to know what blocks in
> the microto supervisor
> because she doesn't
> on an> ice tray and then make a section. That is how I learned to cut
in hi > school. Instead she just trims and cuts the blocks at 4 mic one by one
> using the same blade until it wears out and she coo only
> freezing spray.
>
> She doesn't lik according to her
> is a w > unfort have to go
> without
&g of minutes > sometimes use freeze spray when the blocks get warm but when I cool them
> with ice I don't need to use freeze spray that much. Her works
> but is more successful when the blocks are well pro > difficulty getting completed sections this way and s trying
> to get the perfect section. Sometimes I have other times
> is tedious using this technique. Anoth the blades
> get worn down quicker when you prefer two
> separate blades, one to they stay
> sharp for more >
> She discourages the use of ice but then complains t running out
> of freezing spray for the frozen sections to doesn't make
> sense. It is obvious that if she encou blocks then
> we will be using less freezing >
> Another reason she discourages the use of ice is t are not
> meant to be chilled which is pretty underst small
> biopsies such as gastric and skin and too hard
> and tear off from the block so I breast and
> colon biopsies on ice b tedious to
> cut even when >
>
>
> I want t trim,
> coo time
> c mentioned.
> >
>
> Thanks.
> _______________________________ > Histonet mailing list
> [45]Histon > [46]http: ------- Message: 10
Date: Sat, 29 Sep 2012 09:41: From: "Jackie O'Connor" <[47]b427297 <@t> aol.com>
Subj Freezing
Spray< [49]histonet <@t> lists.utsouthw Message-ID: <8CF6C[50]5F39ED7 501-B8C-4B86D <@t> Webmail-m117.sysops.aol.com>
Content-Type: text/pla It has been my experience that using fr artifacts in the paraffin block as well as the tiss high-throughput lab where all the techs face all their blocks put them on a block of wet ice prior to microtomy. I am not a fan of
f spray.
Jackie O'
-----Original Message-----
From: Jenny Vega <[51]histotech411 <@t> gmail.com>
To: histonet <[52]histonet@ Sent: Fri, Sep 28, 2012 9:40 pm
Subj Spray
I want to know what is your preferred method for cutting paraffin
block the microtome everyday. At work I am having issues with my supervis or
because we have different ways of doing things like for example she d oesn't
like to use the technique where you first trim the tissue, cool i on an
ice tray and then make a section. That is how I learned to cut i histotech
school. Instead she just trims and cuts the blocks at 4 micr by one
using the same blade until it wears out and she cools the freezing spray.
She doesn't like to cool the blocks to her
is a waste of time and that is w unfortunately some blocks are extreme to go
back to my preferred technique. I f wrinkles when I chill and soak the blo minutes. I
sometimes use freeze spray when th them
with ice I don't need to use free works
but is more successful when the difficulty getting completed sections trying
to get the perfect section. Sometime times
is tedious using this technique. A blades
get worn down quicker when you prefer two
separate blades, one to trim they stay
sharp for more time.
< running ou of freezing spray for the frozen sections too quickly which doesn't
ma sense. It is obvious that if she encourages to use ice to cool blocks then
we will be using less freezing spray.
Another reason she di are not
meant to be chilled small
biopsies such as ga hard
and tear off from breast and
colon biopsies tedious to
cut even w I want to know if it's c trim,
cool on ice and section comparing it with other way Thanks.
_______ Histonet mailing list
[53]Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Sat, From: Rene J Buesa <[54]rjbuesa <@t> yahoo.com Subject: Re: [Histonet] Cooling paraffin blocks with ice VS. Fr eezing
Spray
To: Jenny Vega <[55]histotech411 <@t> gmail.com>,
"[56]histonet <@t> lists.utsouthwestern.edu"
<[57]histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<134892771< href="mailto:9.94383.YahooMailNeo <@t> web163104.mail.bf target=_blank>9.94383.YahooMailNeo <@t> web163104.mail.bf1.yahoo. Content-Type: text/plain; charset=utf-8
Jenny:
H Let me go s 1- we always used those gelatin filled trays that are froze from the productivity and quality point of views, it is better to tri m all the blocks one tray first and place them back face down
to 2- after they have been trimed and cooled, you start cutting one one
3- using coolant spray is not advisable because it costs too muc and although the refrigerant is supposed to be innocuous, it could be
a s 4- the best way to handle a difficult block is: trimâ †’cool in a tray→start going deeper to get
the c cube wrapped Your productivity wi → cut each blocks indivi René J.
________________________________From: Jenny Vega
<[58]histotech411 <@t> gmail.com>
To: [59]histonet <@t> lists.utsouthwestern.edu
Sent: Friday, September 28, 20 Subject: [Histonet] Cooling paraffin blocks with ice VS. Fre Spray
I want to know what is your preferred method for cutting blocks in
the microtome everyday. At work I am having issues w supervisor
because we have different ways of doing things like fo doesn't
like to use the technique where you first trim the an
ice tray and then make a section. That is how I l histotech
school. Instead she just trims and cuts the b by one
using the same blade until it wears out an only
freezing spray.
She doesn't like to c to her
is a waste of tim unfortunately some blo to go
back to my preferred without
wrinkles wh minutes. I
sometim them
with i works
but i difficulty time trying< days but other time is tedious using this technique. Another thing I notice is that the
bl get worn down quicker when you use them to trim and section. I pref er two
separate blades, one to trim and the other one to section. I feel they stay
sharp for more time.
She discourages the use of ice bu running out
of freezing spray for the froze make
sense. It is obvious that if s blocks then
we will be using less freez Another reason she discourages the use of ice is that som are not
meant to be chilled which is pretty understandable. I c small
biopsies such as gastric and skin and bone because they hard
and tear off from the block so I avoid that but I pref breast and
colon biopsies on ice because these are fatty tiss tedious to
cut even when relying only on freezing spray.< I want to know if it's completely acceptable for me to prefe trim,
cool on ice and section technique and if you feel is a waste comparing it with other ways of cutting such as the one I menti Thanks.
___________________________________________ Histonet mailing list
[60]Histonet <@t> lists.utsouthwester [61]http://lists.utsouthwestern.edu/ ------------------------------
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