[Histonet] Paraffin and Tissue

Jackie O'Connor b427297 <@t> aol.com
Fri Sep 28 12:05:22 CDT 2012

It has been my experience that tissues that remain in paraffin too long (like over a weekend) become brittle and hard.  If we are embedding over 300 blocks, those blocks may remain in the embedding station for up to 6 hours - but I personally strongly recommend sticking to your SOP for processing.  Besides, if your SOP says paraffin for 3 hours, leaving them longer is a violation of your SOP.
Jackie O'

-----Original Message-----
From: Demetria Ross <deshsmith1 <@t> gmail.com>
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Thu, Sep 27, 2012 5:21 pm
Subject: [Histonet] Paraffin and Tissue

I'm curious to know how long can tissue stay on the machine in paraffin
efore it becomes a problem I have left tissue stay in paraffin 30 min-2
ours before I take it off but not on a daily basis Thanks in advance
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