[Histonet] RE: back up cryostat

Lynette Pavelich LPaveli1 <@t> hurleymc.com
Sat Sep 22 07:33:23 CDT 2012

Our back up died, and administration, in a thought of saving money, decided to not get it repaired as we only get about 5 frozens/week. 
Well, I got called in on a Saturday for a frozen, and, yup, you guessed it.......the brand new one was room temperature!! (housekeeping mistakenly turned it off)
I turned it on, ran and put the blade holder in the -70 freezer in blood bank, the blades in our histobath (isopentane freezer) and prayed for an hour until the frozen came down. I could only get about 2 swipes on the tissue before it started sliding off the chuck! Removed it and re-froze it until I was able to get a section!! What a day. 
Administration decided to repair the back up!! I vote for a back up!


From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Barbara.Crill <@t> LPNT.net [Barbara.Crill <@t> LPNT.net]
Sent: Friday, September 21, 2012 8:06 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] back up cryostat

I would like to know what the consensus is on having a backup cryostat.
Does anyone have a back up cryostat?

I am nervous about not having a backup cryostat

Antoinette Crill

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