[Histonet] burned tissue artifact: need help

[Rene J Buesa]

Unfortunately what you describe is irreversible. Try to get sections, treat them as gently as you can, and move on to the next batch of cases making sure that this does not happen again.Request "understanding" from your pathologists with this batch.
René J.


________________________________
From: "kiran_g <@t> sbcglobal.net" <kiran_g <@t> sbcglobal.net>
To: Amos Brooks <amosbrooks <@t> gmail.com>; histonet-bounces <@t> lists.utsouthwestern.edu; "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Thursday, September 20, 2012 8:37 PM
Subject: Re: [Histonet] burned tissue artifact: need help

Hi all,
Due to water contamination on the processor some of our cases had processing artifact with poor histology. Any suggestion to remedy this issue? We did reprocess the blocks but still not good. Any cutting or staining tips so slides can be readable. 
Thank you all,
Kiran
Sent from my Verizon Wireless BlackBerry

-----Original Message-----
From: Amos Brooks <amosbrooks <@t> gmail.com>
Sender: histonet-bounces <@t> lists.utsouthwestern.edu
Date: Thu, 20 Sep 2012 17:54:04 
To: histonet <@t> lists.utsouthwestern.edu<histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] Picric acid

Hi,
The Halifax explosion was indeed a very dramatic event. Anyone unfamiliar
with the story should certainly read up on it. It was truly incredible. The
link to the Wikipedia article was previously posted. A couple of important
points about this story. The ship that blew up was carrying metric *tons*
of picric acid. The ship also was carrying tons of other explosive material
(nitroglycerine amongst others).
If your lab has tons of picric acid (not 10-100 grams like most labs) and
tons of other explosives, you might have cause for panic. If not, you
likely have an extremely small amount stored under water or in a solution
which poses less risk when used & stored properly than many other chemicals
in a lab.
I don't mean to say there is no risk, but I would say the concern is a bit
overly dramatic. Like a carpenter, know your tools and how to treat them
and they will serve you well. Otherwise ANY of the tools you have are
likely to bite you.

Amos
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