[Histonet] RE: thickness of slides

Vanessa Perez vperez <@t> pathreflab.com
Tue Sep 18 13:26:17 CDT 2012

Routine at 4, lymph nodes/bone marrows at 2, GI/prostate at 3

Prostate we cut 3 shallow levels..... pick up 2 extra slides from the second level as unstained  which get used for p63/PIN cocktail (p504s/p63) if requested....
Each slide has one level...2 sections per slide


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Tuesday, September 18, 2012 1:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] thickness of slides

Can you tell me what thickness you cut your routine slides for H&E and immuno (in particular lymph nodes).  


Also, your protocol for cutting prostate needle core biopsies.....how many spares you cut and do you designate the level on the slide label if multiple levels are submitted on one slide?




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