[Histonet] Cd31

[Jackie O'Connor]

SC had a goat anti mouse CD31 and the goat croaked about 6 years ago.  I have yet to see a better CD31 in FFPE mouse.


-----Original Message-----
From: Patsy Ruegg <pruegg <@t> ihctech.net>
To: helen.ilsley <helen.ilsley <@t> uct.ac.za>; histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Mon, Sep 17, 2012 12:38 pm
Subject: RE: [Histonet] Cd31


Unfortunately we do not have an anti rat cd31 like the rat anti ms one, so
what I have used in rat tissue is either CD34 (not that specific to endo
cells, stains a lot of other stuff), SMA or F8 (these two pick up the more
mature vessels but not the early ones), we need an anti rat CD31 like the
anti ms one from Dianova.  Years ago SC had a good cd31 made in a rabbit or
goat that worked well in ms or rat tissue but the rabbit died or something
and we have not gotten another good one yet for rat tissue that I know of???

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net 
www.ihcrg.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of helen.ilsley
Sent: Saturday, September 15, 2012 2:15 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Cd31

Hi

Which endothelial marker would you use for rat tissue?
I am really interested to see what you all say.
Thanks in advance
Helen

Sent from my BlackBerryR wireless device


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