[Histonet] cell membrane

Fri Sep 14 16:08:54 CDT 2012

Liz,
It is possible to play with different catenins (gamma etc.) to get rid of nuclear staining. But if the target is epithelium I would try to use EpCAM first. And in case of different epithelial tumors EpCAM usually is up-regulated.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: AGleiberman <@t> cbiolabs.com

-----Original Message-----
From: Elizabeth Chlipala [mailto:liz <@t> premierlab.com] 
Sent: Friday, September 14, 2012 4:08 PM
To: Anatoli Gleiberman; pruegg <@t> ihctech.net
Subject: RE: [Histonet] cell membrane

Thanks Anatoli

We have worked with beta-catenin before (4 different sources of antibody) and in the tissue that we looked at (tumor) we saw a combination of membrane, cytoplasmic and nuclear staining.  I went back to some of the images from the phalloidin staining and cell membrane staining was variable, some cells were outlined nicely others were not.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: Anatoli Gleiberman [mailto:AGleiberman <@t> cbiolabs.com]
Sent: Friday, September 14, 2012 12:41 PM
To: Elizabeth Chlipala; pruegg <@t> ihctech.net
Subject: RE: [Histonet] cell membrane

Liz, Patsy,

Phalloidin will definitely stain under membrane actin in liver cells - but for all other cell types it will stain all intra-cytoplasmic actin stress fibrils and cytoplasmic actin network. For many epithelial cells with exclusion  of keratinocytes and hepatocytes the best membrane marker is EpCAM. There are rat anti-mouse EpCAM antibody and mouse anti-human EpCAM antibody commercially available. It is possible to use anti-beta-catenin antibody for most of epithelia. So, combination of EpCAM and beta-catenin will stain on sections cell membranes of all epithelia. However, there are no satisfactory staining for cell membranes of connective tissue and smooth muscle cells and I am not sure about neurons and skeletal muscle cells. I am afraid, there are no such things as one reagent staining exclusively cell membranes of all cell types.

Anatoli Gleiberman, PhD
Director of Histopathology
Cleveland Biolabs, Inc
73 High Street
Buffalo, NY 14203
phone:716-849-6810 ext.354
fax:716-849-6817
e-mail: AGleiberman <@t> cbiolabs.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala
Sent: Friday, September 14, 2012 10:43 AM
To: 'Patsy Ruegg'; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] cell membrane

Patsy

Not sure if this will work, but I remember a project we did on fixed vibratome sections of mouse liver.  We did a bunch of IF markers I think we used Phalloidin and that seemed to outline the liver cells nicely.  We used it already conjugated.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Thursday, September 13, 2012 12:06 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cell membrane

Dear Histochemist,

Does anyone know of a stain or histochemical reaction which will exclusively stain cell membrane and no other parts of the cell or other tissue components?

Best regards,

Patsy

Patsy Ruegg, HT(ASCP)QIHC
Director of Histology and IHC

IHCtech a subsidiary of Flagship Bio-Sciences, LLC Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80045

P-720-859-4060
F-720-859-4110
email  <mailto:pruegg <@t> ihctech.net> pruegg <@t> ihctech.net & email  <mailto:pruegg <@t> flagshipbio.com> pruegg <@t> flagshipbio.com

web site  <http://www.ihctech.net> www.ihctech.net

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet