[Histonet] endothelial cell marker

[Patsy Ruegg]

By far the best ab for endothelial cells in mouse tissue is rat anti CD31
from Dianovo, it picks up the really early forming vessels better than any
other cd31 or F8 or SMA I have ever used.  We like it in AP/red.

Regards,
Patsy


Hi,
     My favorite by far is VonWillebrandt (spelling?) factor VIII. Dako has
a really good one (Cat# A0082) that works great in mouse tissue. CD31 is OK
but it is really finicky. CD34 works but F8 is easier and more reliable.

Amos


On Wed, Sep 5, 2012 at 1:01 PM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:

> Message: 5
> Date: Wed, 5 Sep 2012 10:04:58 +0000
> From: "Bruijntjes, J.P. (Joost)" <joost.bruijntjes <@t> tno.triskelion.nl>
> Subject: [Histonet] endothelial cell marker
> To: "Histonet <@t> lists.utsouthwestern.edu"
>         <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>         <A3A9D8B3F110D44FA1022B49913668668835BE43 <@t> EXC-MBX03.tsn.tno.nl>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi all
>
> Is anyone of you familiar with an antibody directed against endothelial
> cells which can be applied on FFPE mouse tissues?
>
> Best regards
> Joost Bruijntjes
>
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