[Histonet] RE: ] Eosin and the Peloris

[Mayer,Toysha N]

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If you don't want eosin in the alcohols, try tinting your formalin with hematoxylin.  When I worked at LSU, one of the techs put hematoxylin in the formalin that the tissues sat in before going on the processor, after grossing.  It gave a light tint to the tissue, but did not interfere with anything else.  It was not applied directly to the tissue, just the formalin used that day. Eosin in the formalin would not work, a more water based stain would.

Hope that helps.

Toysha

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Date: Sat, 8 Sep 2012 11:35:46 -0700
From: Madeleine Huey <madeleinehuey <@t> gmail.com>
Subject: [Histonet] Re: Histonet Digest, Vol 106, Issue 7
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Date: Fri, 7 Sep 2012 10:22:26 -0700 (PDT)
From: angela smith <we3smitty <@t> yahoo.com>
Subject: [Histonet] Eosin and the Peloris
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I know the original Peloris states that eosin should not be used in the alcohols It does say you can put it in the formalins.

What are you all doing out there to mark your small needle bx and gi bx's?  We prefer methods where we add to the reagents on the processor.

We have tried just formalin with eosin and eosin phlox. and it does not do a very good job.

thanks


Angela,
We add Eosin in our most purity alcohol bottles (2 bottles with ~ 95%
- 100% ETOH) & they work great.  I do not recommend it in Formalin, because the Eosin tend to wash out during the dehydration process.
No loss of small biopsies in my lab yet.
Good Luck!
Madeleine Huey BS, HTL (ASCP) QIHC
Supervisor - Pathology (IPOX & Histology) madeleine_h <@t> elcaminohospital.org