[Histonet] IHC- phospho-JNK.

Thotakura, Anil K a.thotakura <@t> imperial.ac.uk
Thu Sep 6 14:10:55 CDT 2012

Hi All,

i need you guys help.

I am doing IHC on 4% formalin fixed mice liver paraffins section and want to stain for Phospho-JNK. I am using primary Antibody from cell signaling ( Cat.no-9251). i followed the protocol exactly same as they suggested but i did't see any staining. I did antigen retrieval using Na citrate pH6.0.

please give some input to refine my technique.

Many Thanks,

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