[Histonet] IHC- phospho-JNK.

[Thotakura, Anil K]

Hi All,

i need you guys help.

I am doing IHC on 4% formalin fixed mice liver paraffins section and want to stain for Phospho-JNK. I am using primary Antibody from cell signaling ( Cat.no-9251). i followed the protocol exactly same as they suggested but i did't see any staining. I did antigen retrieval using Na citrate pH6.0.

please give some input to refine my technique.

Many Thanks,
Kumar.