[Histonet] IHC- phospho-JNK.
Thotakura, Anil K
a.thotakura <@t> imperial.ac.uk
Thu Sep 6 14:10:55 CDT 2012
i need you guys help.
I am doing IHC on 4% formalin fixed mice liver paraffins section and want to stain for Phospho-JNK. I am using primary Antibody from cell signaling ( Cat.no-9251). i followed the protocol exactly same as they suggested but i did't see any staining. I did antigen retrieval using Na citrate pH6.0.
please give some input to refine my technique.
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