[Histonet] RE: ImageJ : start help for muscle fiber count

[Elizabeth Chlipala]

Tora

If you are using an H&E it was vertually impossible in our hands to try to threshold fiber area.  We worked with Image Pro Plus for about 6 months trying to get this to work and we could not, we ended up creating an algorithm in image pro that allowed us to trace the fibers and that would calculate fiber area and diameter, etc.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
liz <@t> premierlab.com

Ship to address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tora Bardal [tora.bardal <@t> bio.ntnu.no]
Sent: Thursday, September 06, 2012 5:48 AM
To: mail=histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] ImageJ : start help for muscle fiber count

Any guru out there that might give me some ideas how to measure size and number of muscle fibers in a cross section using ImageJ
Area by using freehand tool  works................. but I'm having problems making a threshold with the muscle cells outlined for automatic measurements.
 I'm not experienced, might be I haven't found the correct tool :-) Picture sample:
http://www.nt.ntnu.no/users/tbardal/muscle/muscle%20paraffin%202%c2%b5%2016x.jpg

_________________________________________________________________________________
Tora Bardal
Overingeniør/ Senior Engineer
NTNU Senter for fiskeri og havbruk /NTNU Center of Fisheries and Aquaculture NTNU, 7491 Trondheim



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