[Histonet] CD31 for FFPE IHC on Mouse

[Kim Merriam]

I second the Dianova antibody - we have had very good luck with it.  Standard citrate HIER works for us.
 


Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
 

________________________________
 From: Patsy Ruegg <pruegg <@t> ihctech.net>
To: 'Mark Elliott' <Mark.Elliott <@t> hli.ubc.ca>; histonet <@t> lists.utsouthwestern.edu 
Sent: Sunday, October 21, 2012 11:20 AM
Subject: RE: [Histonet] CD31 for FFPE IHC on Mouse
  
Rat anti mouse cd31 from Dionova is the way to go it is better than all the
others.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pruegg <@t> ihctech.net
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mark Elliott
Sent: Friday, October 19, 2012 3:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD31 for FFPE IHC on Mouse

Amy
Can you please share the responses you got with the rest of us
Thanks
Mark


Message: 8
Date: Thu, 18 Oct 2012 16:21:55 -0400
From: "Amy Porter" <portera <@t> msu.edu>
Subject: RE: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse
Model
To: "'Amy Porter'" <portera <@t> msu.edu>,"'Histonet'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <003101cdad6e$3792fc80$a6b8f580$@edu>
Content-Type: text/plain;charset="US-ASCII"

Thanks to all for responses......looks like most roads lead to once place
which is spectacular!!  Just what I needed.  Amy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amy Porter
Sent: Thursday, October 18, 2012 3:36 PM
To: 'Histonet'
Subject: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse Model

Anyone out there have CD31 working well on FFPE samples for Mouse Samples??
I know this might be a long shot, however I haven't looked for anything on
this in quite awhile.



Amy S. Porter, HT(ASCP) QIHC

Michigan State University

Investigative HistoPathology Laboratory

William S. Spielman, Ph.D. - Director

Patricia K. Senagore, M.D. - Consulting Pathologist

Department of Physiology / Human Pathology

Biomedical Physical Sciences Building 

567 Wilson Road - Room 2133

East Lansing, MI  48824-3320

Phone:  517-884-5026

Fax:  517-432-1368

portera <@t> msu.edu

http://www.humanpathology.msu.edu/




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