[Histonet] CD4 T cells FFPE vs frozen

Kim O'Sullivan kim.osullivan <@t> monash.edu
Tue Oct 23 22:16:15 CDT 2012


I am aware that there are mouse anti human CD4 markers for the detection of
CD4 T cells in human FFPE tissue. My question is do these antibodies (mouse
clone 1F6 and 4B12) detect the same number of T cells that are detected in
frozen tissue? Secondly does anyone know of any literature that validates
or tests this? This question arises out of the discrepany I have noticed in
our area of research(glomerulonephritis) in literature on the reported
number of T cells- papers using frozen tissue report 3 times the amount of
cells in the same disease studied on FFPE tissue.

Any help or any anectodal evidence would be appreciated.

Kim O'Sullivan

Kim O'Sullivan
Centre for Inflammatory Diseases
Department of Medicine
Southern Clinical School
Monash Medical Centre
Level 5, Block E
246 Clayton Road
Clayton, Melbourne
VIC 3168

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