[Histonet] CD4 T cells FFPE vs frozen

[Kim O'Sullivan]

Hi,

I am aware that there are mouse anti human CD4 markers for the detection of
CD4 T cells in human FFPE tissue. My question is do these antibodies (mouse
clone 1F6 and 4B12) detect the same number of T cells that are detected in
frozen tissue? Secondly does anyone know of any literature that validates
or tests this? This question arises out of the discrepany I have noticed in
our area of research(glomerulonephritis) in literature on the reported
number of T cells- papers using frozen tissue report 3 times the amount of
cells in the same disease studied on FFPE tissue.

Any help or any anectodal evidence would be appreciated.

Kim O'Sullivan

-- 
Kim O'Sullivan
Centre for Inflammatory Diseases
Department of Medicine
Southern Clinical School
Monash Medical Centre
Level 5, Block E
246 Clayton Road
Clayton, Melbourne
VIC 3168
Australia
Ph:+61395945549
Fax:+6195946495