[Histonet] reply to message 7
M. Kap
m.kap.1 <@t> erasmusmc.nl
Mon Oct 22 14:00:31 CDT 2012
Hi there,
Just take dog's appendix or lymphnode and titrate the antibody until it looks like the reference on www.cqpath.com
good luck!
Marcel
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Sent: Monday, October 22, 2012 7:08 PM
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Subject: Histonet Digest, Vol 107, Issue 28
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Today's Topics:
1. RE: CD31 for FFPE IHC on Mouse (Amy Porter)
2. Renal biopsies and muscle biopsies (Vickroy, Jim)
3. Need help with spirochete control (Sheila Fonner)
4. RE: Renal biopsies and muscle biopsies (Lynette Pavelich)
5. RE: Need help with spirochete control (Lynette Pavelich)
6. RE: Renal biopsies and muscle biopsies (Morken, Timothy)
7. Dako cd3 and cd79a (Stuart Beaver)
8. RE: Dako cd3 and cd79a (Amy Porter)
----------------------------------------------------------------------
Message: 1
Date: Mon, 22 Oct 2012 08:24:10 -0400
From: "Amy Porter" <portera <@t> msu.edu>
Subject: RE: [Histonet] CD31 for FFPE IHC on Mouse
To: "'Patsy Ruegg'" <pruegg <@t> ihctech.net>, "'Mark Elliott'"
<Mark.Elliott <@t> hli.ubc.ca>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001801cdb050$241b8cf0$6c52a6d0$@edu>
Content-Type: text/plain; charset="us-ascii"
This antibody was the overwhelming consensus out of all the information I
received. Thanks to all for the input!! Amy
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Sunday, October 21, 2012 11:20 AM
To: 'Mark Elliott'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] CD31 for FFPE IHC on Mouse
Rat anti mouse cd31 from Dionova is the way to go it is better than all the
others.
Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting, LLC
40864 Arkansas Ave
Bennett, CO 80102
Phone: 303-644-4538
Fax: 720-859-4110
pruegg <@t> ihctech.net
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mark Elliott
Sent: Friday, October 19, 2012 3:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD31 for FFPE IHC on Mouse
Amy
Can you please share the responses you got with the rest of us Thanks Mark
Message: 8
Date: Thu, 18 Oct 2012 16:21:55 -0400
From: "Amy Porter" <portera <@t> msu.edu>
Subject: RE: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse Model
To: "'Amy Porter'" <portera <@t> msu.edu>,"'Histonet'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <003101cdad6e$3792fc80$a6b8f580$@edu>
Content-Type: text/plain;charset="US-ASCII"
Thanks to all for responses......looks like most roads lead to once place
which is spectacular!! Just what I needed. Amy
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amy Porter
Sent: Thursday, October 18, 2012 3:36 PM
To: 'Histonet'
Subject: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse Model
Anyone out there have CD31 working well on FFPE samples for Mouse Samples??
I know this might be a long shot, however I haven't looked for anything on
this in quite awhile.
Amy S. Porter, HT(ASCP) QIHC
Michigan State University
Investigative HistoPathology Laboratory
William S. Spielman, Ph.D. - Director
Patricia K. Senagore, M.D. - Consulting Pathologist
Department of Physiology / Human Pathology
Biomedical Physical Sciences Building
567 Wilson Road - Room 2133
East Lansing, MI 48824-3320
Phone: 517-884-5026
Fax: 517-432-1368
portera <@t> msu.edu
www.humanpathology.msu.edu
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------------------------------
Message: 2
Date: Mon, 22 Oct 2012 07:58:12 -0500
From: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
Subject: [Histonet] Renal biopsies and muscle biopsies
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BB0B9F1A8373F14FA2974E8CB24BF9CF049C60F6 <@t> mmc-mail.ad.mhsil.com>
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I have been at the same hospital for over 34 years. I have seen pathologists come and go, and have seen changes in services like everyone has, some good and some bad. Since workflow changes demands that we do more with less, we are evaluating some of the extra things we do to see if some of these duties need to be done by other staff and not histotechs.
In our hospital whenever a renal biopsy procedure is done in ultrasound they call the histology department and one of the staff go to the ultrasound and receive the tissue from the radiologist. The tech then brings the tissue to the gross room and the grossing staff separates and processes the specimen. In the old days ultrasound would page the renal pathologist and the renal pathologist would go get the specimens and then bring them to the gross lab, but it seems like many things this has now been relegated to the technical staff. We also pick up renal biopsies and muscle biopsies specimens from another hospital in the same town that sends us renal biopsies since we have the local renal pathologist.
We do not routinely pick up any other specimens because we have a courier system, but the renal and sometimes muscle biopsy service still remains in the hands of the histology department. I would like to know how other hospitals handle transportation of specialized specimens such as renal biopsies and muscle biopsies. In the old days there was a purpose for a pathologist to be present since often the radiologist asked the pathologist to evaluate if he or she had enough adequate tissue, but today with better ultrasound methods rarely do the radiologists ask the techs to evaluate whether enough adequate tissue has been taken.
I need some background information from others before I approach the powers to be to come up with an alternate plan so that the histotechs can spend more time in the lab and less time being a courier service for these biopsies. Of course an alternate way will have to include a method that is reliable and not threaten the patient results.
Your thoughts?
James Vickroy BS, HT(ASCP)
Surgical and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046
________________________________
This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited.
------------------------------
Message: 3
Date: Mon, 22 Oct 2012 06:17:35 -0700 (PDT)
From: Sheila Fonner <fourfonners <@t> yahoo.com>
Subject: [Histonet] Need help with spirochete control
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1350911855.27329.YahooMailNeo <@t> web31915.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello all,
I am in need of some help with a spirochete protocol for the Leica Bond.? I am using a Biocare primary antibody.? I've tried ER1 for 20 min. with 15 min. Ab with no luck.? Does anyone do this, and do you think increasing the primary Ab time would help?? I've used this same Ab before on a different platform and had no problem.? Any help or advice would be greatly appreciated.? Thanks.
Sheila, HT (ASCP)
------------------------------
Message: 4
Date: Mon, 22 Oct 2012 13:27:55 +0000
From: Lynette Pavelich <LPaveli1 <@t> hurleymc.com>
Subject: [Histonet] RE: Renal biopsies and muscle biopsies
To: "Vickroy, Jim" <vickroy.jim <@t> mhsil.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<89F4666A496DC949A819ECC40E11C867BF564A9F <@t> EXCHANGEMB1.hmc.hurleymc.com>
Content-Type: text/plain; charset="us-ascii"
In our hospital, the radiology techs are responsible for bringing the renal biopsies up to the department (in saline) as soon as the procedure is obtained. They show the specimen to the attending pathologist, who signs off on the radiology record as sufficient. Then the histotechs divide the specimen for the EM, IF and NBF specimens.
Our muscle/nerve biopsies are all done in-house up in OR and are sent directly to the lab in a dumbwaiter "fresh" on saline-soaked gauze where the histotechs then divide up the specimen according to protocol. The nurses are responsible for delivery to the histology department.
hope this helps,
Lynette
Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI 48503
ph: 810.262.9948
mobile: 810.444.7966
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Vickroy, Jim [Vickroy.Jim <@t> mhsil.com]
Sent: Monday, October 22, 2012 8:58 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Renal biopsies and muscle biopsies
I have been at the same hospital for over 34 years. I have seen pathologists come and go, and have seen changes in services like everyone has, some good and some bad. Since workflow changes demands that we do more with less, we are evaluating some of the extra things we do to see if some of these duties need to be done by other staff and not histotechs.
In our hospital whenever a renal biopsy procedure is done in ultrasound they call the histology department and one of the staff go to the ultrasound and receive the tissue from the radiologist. The tech then brings the tissue to the gross room and the grossing staff separates and processes the specimen. In the old days ultrasound would page the renal pathologist and the renal pathologist would go get the specimens and then bring them to the gross lab, but it seems like many things this has now been relegated to the technical staff. We also pick up renal biopsies and muscle biopsies specimens from another hospital in the same town that sends us renal biopsies since we have the local renal pathologist.
We do not routinely pick up any other specimens because we have a courier system, but the renal and sometimes muscle biopsy service still remains in the hands of the histology department. I would like to know how other hospitals handle transportation of specialized specimens such as renal biopsies and muscle biopsies. In the old days there was a purpose for a pathologist to be present since often the radiologist asked the pathologist to evaluate if he or she had enough adequate tissue, but today with better ultrasound methods rarely do the radiologists ask the techs to evaluate whether enough adequate tissue has been taken.
I need some background information from others before I approach the powers to be to come up with an alternate plan so that the histotechs can spend more time in the lab and less time being a courier service for these biopsies. Of course an alternate way will have to include a method that is reliable and not threaten the patient results.
Your thoughts?
James Vickroy BS, HT(ASCP)
Surgical and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046
________________________________
This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited.
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------------------------------
Message: 5
Date: Mon, 22 Oct 2012 13:42:01 +0000
From: Lynette Pavelich <LPaveli1 <@t> hurleymc.com>
Subject: RE: [Histonet] Need help with spirochete control
To: Sheila Fonner <fourfonners <@t> yahoo.com>, Histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<89F4666A496DC949A819ECC40E11C867BF564AC4 <@t> EXCHANGEMB1.hmc.hurleymc.com>
Content-Type: text/plain; charset="us-ascii"
Hi Sheila,
We also use the Leica Bond using the Biocare antibody. We have had success using the RED detection kit for the spirochete with this protocol: HIER 1 (6.0) for 20 minutes and 30 minutes in the antibody.
hopes this helps,
Lynette
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Sheila Fonner [fourfonners <@t> yahoo.com]
Sent: Monday, October 22, 2012 9:17 AM
To: Histonet
Subject: [Histonet] Need help with spirochete control
Hello all,
I am in need of some help with a spirochete protocol for the Leica Bond. I am using a Biocare primary antibody. I've tried ER1 for 20 min. with 15 min. Ab with no luck. Does anyone do this, and do you think increasing the primary Ab time would help? I've used this same Ab before on a different platform and had no problem. Any help or advice would be greatly appreciated. Thanks.
Sheila, HT (ASCP)
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 6
Date: Mon, 22 Oct 2012 16:03:13 +0000
From: "Morken, Timothy" <Timothy.Morken <@t> ucsfmedctr.org>
Subject: [Histonet] RE: Renal biopsies and muscle biopsies
To: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <761E2B5697F795489C8710BCC72141FF029413 <@t> ex07.net.ucsf.edu>
Content-Type: text/plain; charset=us-ascii
James,
At our institution the techs do not go to kidney or muscle bx. The resident will deliver the fresh specimen to either directly to our lab or to grossing. Hospitals send them in fixative and transport media that we provide to them.
My feeling is that if the techs are not there to check the adequacy of the specimen then there is no need for them to go. We used to do that routinely to ensure good material. These days we occasionally get an inadequate biopsy but considering we do over 700 renal and 200 muscle per year it is a very low percentage of the total and does not justify tech time to examine each one.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
505 Parnassus Ave, Box 1656
Room S570
San Francisco, CA 94143
(415) 353-1266 (ph)
(415) 514-3403 (fax)
tim.morken <@t> ucsfmedctr.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Monday, October 22, 2012 5:58 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Renal biopsies and muscle biopsies
I have been at the same hospital for over 34 years. I have seen pathologists come and go, and have seen changes in services like everyone has, some good and some bad. Since workflow changes demands that we do more with less, we are evaluating some of the extra things we do to see if some of these duties need to be done by other staff and not histotechs.
In our hospital whenever a renal biopsy procedure is done in ultrasound they call the histology department and one of the staff go to the ultrasound and receive the tissue from the radiologist. The tech then brings the tissue to the gross room and the grossing staff separates and processes the specimen. In the old days ultrasound would page the renal pathologist and the renal pathologist would go get the specimens and then bring them to the gross lab, but it seems like many things this has now been relegated to the technical staff. We also pick up renal biopsies and muscle biopsies specimens from another hospital in the same town that sends us renal biopsies since we have the local renal pathologist.
We do not routinely pick up any other specimens because we have a courier system, but the renal and sometimes muscle biopsy service still remains in the hands of the histology department. I would like to know how other hospitals handle transportation of specialized specimens such as renal biopsies and muscle biopsies. In the old days there was a purpose for a pathologist to be present since often the radiologist asked the pathologist to evaluate if he or she had enough adequate tissue, but today with better ultrasound methods rarely do the radiologists ask the techs to evaluate whether enough adequate tissue has been taken.
I need some background information from others before I approach the powers to be to come up with an alternate plan so that the histotechs can spend more time in the lab and less time being a courier service for these biopsies. Of course an alternate way will have to include a method that is reliable and not threaten the patient results.
Your thoughts?
James Vickroy BS, HT(ASCP)
Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center
217-788-4046
________________________________
This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited.
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------------------------------
Message: 7
Date: Mon, 22 Oct 2012 17:22:32 +0100
From: Stuart Beaver <sbeaver0526 <@t> hotmail.co.uk>
Subject: [Histonet] Dako cd3 and cd79a
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BLU401-EAS4590F55447AF7FDC42F3B56E77A0 <@t> phx.gbl>
Content-Type: text/plain; charset="utf-8"
Hi all,
I am looking to for approximate dilution ranges for Dako cd3 and cd79a in canine lymph node samples for T and Bcell Lymphoma. I am using the Flex mini kit at the moment with high pH antigen retrieval.
Regards
Stuart
------------------------------
Message: 8
Date: Mon, 22 Oct 2012 12:52:55 -0400
From: "Amy Porter" <portera <@t> msu.edu>
Subject: RE: [Histonet] Dako cd3 and cd79a
To: "'Stuart Beaver'" <sbeaver0526 <@t> hotmail.co.uk>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000601cdb075$aee88a70$0cb99f50$@edu>
Content-Type: text/plain; charset="utf-8"
Don't know what a Flex mini kit is - however we use Dako CD3 @ 1:100 for 60 minute / Heat retrieval steamer - ph 9.0 Tris EDTA / Vector secondaries and enzyme label HRP
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Stuart Beaver
Sent: Monday, October 22, 2012 12:23 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Dako cd3 and cd79a
Hi all,
I am looking to for approximate dilution ranges for Dako cd3 and cd79a in canine lymph node samples for T and Bcell Lymphoma. I am using the Flex mini kit at the moment with high pH antigen retrieval.
Regards
Stuart
------------------------------
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