[Histonet] HSV1/2 controls or virus controls in general

Wed Oct 17 14:36:21 CDT 2012

Hi histonetters,

We formed an alliance with our virology department. They use virus cell culture to determine virus titers and such, so I came up with the idea to let them culture loads of cells, infect them with all kinds of viruses and than simply make paraffin blocks of the cells. 

They use standard methods, so very constant viral loads, so very standard controls... We have great results with BK-virus and Influenza virus so far. Guess it can be done for HSV1/2 also.

Not sure if the site is updated yet,since we only recently started doing this, but check out www.cqpath.com for control tissues et cetera.

Ciao for now,

Marcel
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Sent: Wednesday, October 17, 2012 7:03 PM
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Subject: Histonet Digest, Vol 107, Issue 23

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Today's Topics:

   1. Cytotechnologist Opening (Brannon Owens)
   2. RE: Dako HercepTest Kit (Mark Turner)
   3. HSV I and II control (Coppin, Margaret)
   4. validation Form  (tahseen <@t> brain.net.pk)
   5. Stain for HP (Diana McCaig)
   6. RE: Stain for HP (Weems, Joyce K.)
   7. RE: Stain for HP (Amy Porter)
   8. RE: Stain for HP (Sebree Linda A)
   9. FW: Stain for HP (Vanessa Perez)
  10. RE: Stain for HP (Tom McNemar)
  11. Re: Stain for HP (Rene J Buesa)
  12. RE: Stain for HP (SimmsColon, Janine)
  13. Job opening in Houston, Texas (Remy Lopez)

----------------------------------------------------------------------

Message: 1
Date: Tue, 16 Oct 2012 14:09:43 -0400
From: Brannon Owens <brannon <@t> alliedsearchpartners.com>
Subject: [Histonet] Cytotechnologist Opening
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <CCA31B27.2245F%brannon <@t> alliedsearchpartners.com>
Content-Type: text/plain;       charset="US-ASCII"

Position:  Cytotechnologist
Location:  Port Chester, NY area
Shift:  Monday-Friday Day Shift
To be considered email an updated resume to brannon <@t> alliedsearchpartners.com
To view a complete list of Allied Search Partners current openings go to:
http://www.alliedsearchpartners.com/careers.php
--
Brannon Owens
Recruitment Manager
Allied Search Partners

------------------------------

Message: 2
Date: Tue, 16 Oct 2012 14:34:28 -0400
From: "Mark Turner" <mturner <@t> CSILaboratories.com>
Subject: RE: [Histonet] Dako HercepTest Kit
To: "Cindy Bulmer" <cjbulmer <@t> sbcglobal.net>,    "Histonet"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <467983BDAC5880438E2202F6B9BF79A8CC23EC <@t> exchange-be-01.CSI-LABS.local>
Content-Type: text/plain;       charset="iso-8859-1"

We also Decided to maintain the negative control on our HercepTest assays for this very reason.

Mark Turner, HT(ASCP) QIHC
CSI Laboratories
IHC / Histology Manager

678-319-3321 Direct
770-508-7644 Cell???????????????????????
678-319-1454 Fax
mailto:mturner <@t> csilaboratories.com
csilaboratories.com

2580 Westside Parkway
Alpharetta, GA  30004

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-----Original Message-----
From: Cindy Bulmer [mailto:cjbulmer <@t> sbcglobal.net]
Sent: Tuesday, October 16, 2012 12:18 PM
To: Histonet
Subject: [Histonet] Dako HercepTest Kit

Hello Histonet,

Regarding the negative reagent control on the HercepTest Kit from Dako, order # K5207.? We wrote CAP about this and this was their reply:

CAP would still require the laboratory to abide by any manufacturer instructions that may be more stringent than the CAP requirement.

Since this kit is FDA approved, and the negative control reagent is included in the kit, our facility has decided to continue to do the negative reagent control.

Hope this helps,

Cindy

Cynthia Bulmer HT(ASCP)QIHC

IHC Supervisor, CTPL

Waco, TX
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Message: 3
Date: Tue, 16 Oct 2012 22:34:12 +0000
From: "Coppin, Margaret" <COPPINM <@t> aruplab.com>
Subject: [Histonet] HSV I and II control
To: "'Histonet <@t> lists.utsouthwestern.edu'"
        <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <448AA3F629599F4097B1E11A178B6C06A36760 <@t> EXMBX1.aruplab.net>
Content-Type: text/plain; charset="us-ascii"

Hello everyone,

Does anyone out there have some good HSV control tissue they could share with us? We have a lot of control tissue that we could offer as a trade if interested. Just let me know what you need.

We have tried using the NSH tissue control bank, but have had no luck.

Thanks.

Margaret G. Coppin, HT(ASCP)
Technical Supervisor,
Immunohistochemistry

ARUP Laboratories
500 Chipeta Way
Salt Lake City, UT 84108
phone: (801)583-2787 X3869
email: coppinm <@t> aruplab.com

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Message: 4
Date: Wed, 17 Oct 2012 17:41:28 +0500 (PKT)
From: tahseen <@t> brain.net.pk
Subject: [Histonet] validation Form
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <14058.203.135.35.66.1350477688.squirrel <@t> brain.net.pk>
Content-Type: text/plain;charset=iso-8859-1

Good Morning Histoland,
I was wondering if anyone has a instument and antibody validation Form
they would be
willing to share?

Thanks,
Tahseen

------------------------------

Message: 5
Date: Wed, 17 Oct 2012 15:03:50 +0000
From: Diana McCaig <dmccaig <@t> ckha.on.ca>
Subject: [Histonet] Stain for HP
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <D8866AEBD70A70408259C98EB75DFA72012601 <@t> EXMBX1.esclhin.on.ca>
Content-Type: text/plain; charset="us-ascii"

What stain are you using for HP?--Giemsa. Warthin Starry or IHC.
Do you do them routinely or only when requested?
Are they done on an autostainer or with a kit?

Diana

------------------------------

Message: 6
Date: Wed, 17 Oct 2012 15:13:18 +0000
From: "Weems, Joyce K." <Joyce.Weems <@t> emoryhealthcare.org>
Subject: [Histonet] RE: Stain for HP
To: "'Diana McCaig'" <dmccaig <@t> ckha.on.ca>, Histonet
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <E3A4EBD57A691646BCCED4AA5911A030667C4897 <@t> e14mbx12n.Enterprise.emory.net>

Content-Type: text/plain; charset="us-ascii"

We do IHC routinely.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.weems <@t> emoryhealthcare.org

www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Wednesday, October 17, 2012 11:04 AM
To: Histonet
Subject: [Histonet] Stain for HP

What stain are you using for HP?--Giemsa. Warthin Starry or IHC.
Do you do them routinely or only when requested?
Are they done on an autostainer or with a kit?

Diana

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Message: 7
Date: Wed, 17 Oct 2012 11:17:10 -0400
From: "Amy Porter" <portera <@t> msu.edu>
Subject: RE: [Histonet] Stain for HP
To: "'Diana McCaig'" <dmccaig <@t> ckha.on.ca>,      "'Histonet'"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001201cdac7a$7a6d8d80$6f48a880$@edu>
Content-Type: text/plain;       charset="US-ASCII"

Anatech Ltd here in Michigan has a very nice stain for HP  I think it is
only a couple of components that can be purchased by the quart or the
gallon.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Wednesday, October 17, 2012 11:04 AM
To: Histonet
Subject: [Histonet] Stain for HP

What stain are you using for HP?--Giemsa. Warthin Starry or IHC.
Do you do them routinely or only when requested?
Are they done on an autostainer or with a kit?

Diana

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------------------------------

Message: 8
Date: Wed, 17 Oct 2012 15:17:23 +0000
From: Sebree Linda A <LSebree <@t> uwhealth.org>
Subject: [Histonet] RE: Stain for HP
To: 'Diana McCaig' <dmccaig <@t> ckha.on.ca>, Histonet
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <77DD817201982748BC67D7960F2F76AFD552 <@t> UWHC-MBX12.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"

H. Pylori by IHC; automated.  I believe they're pretty much routinely ordered; sure seems like it anyway!

Linda

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Wednesday, October 17, 2012 10:04 AM
To: Histonet
Subject: [Histonet] Stain for HP

What stain are you using for HP?--Giemsa. Warthin Starry or IHC.
Do you do them routinely or only when requested?
Are they done on an autostainer or with a kit?

Diana

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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 9
Date: Wed, 17 Oct 2012 10:21:28 -0500
From: Vanessa Perez <vperez <@t> pathreflab.com>
Subject: [Histonet] FW: Stain for HP
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <C80B5EE351AAD74DADAB70FABCB78D193BCAB62CA6 <@t> exchange.pasa.local>
Content-Type: text/plain; charset="us-ascii"

IHC on ventana instruments, automatically done on stomach,antrum bx...and as requested

Vanessa

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Wednesday, October 17, 2012 10:04 AM
To: Histonet
Subject: [Histonet] Stain for HP

What stain are you using for HP?--Giemsa. Warthin Starry or IHC.
Do you do them routinely or only when requested?
Are they done on an autostainer or with a kit?

Diana

_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
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------------------------------

Message: 10
Date: Wed, 17 Oct 2012 11:27:02 -0400
From: Tom McNemar <TMcNemar <@t> lmhealth.org>
Subject: [Histonet] RE: Stain for HP
To: 'Diana McCaig' <dmccaig <@t> ckha.on.ca>, Histonet
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <E9A90E28259D2F4E84308C5E8EA8F7B40135569497EC <@t> lmhs-exchange.lmhealth.org>

Content-Type: text/plain; charset="us-ascii"

IHC on the Benchmark.  We routinely do them on all stomach biopsies.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Wednesday, October 17, 2012 11:04 AM
To: Histonet
Subject: [Histonet] Stain for HP

What stain are you using for HP?--Giemsa. Warthin Starry or IHC.
Do you do them routinely or only when requested?
Are they done on an autostainer or with a kit?

Diana

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This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.

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Message: 11
Date: Wed, 17 Oct 2012 08:40:58 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Stain for HP
To: Diana McCaig <dmccaig <@t> ckha.on.ca>,  Histonet
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <1350488458.44817.YahooMailNeo <@t> web163101.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

IHC, when requested, on autostainer
Ren? J.

________________________________
From: Diana McCaig <dmccaig <@t> ckha.on.ca>
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, October 17, 2012 11:03 AM
Subject: [Histonet] Stain for HP

What stain are you using for HP?--Giemsa. Warthin Starry or IHC.
Do you do them routinely or only when requested?
Are they done on an autostainer or with a kit?

Diana

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 12
Date: Wed, 17 Oct 2012 11:46:26 -0400
From: "SimmsColon, Janine" <Janine.SimmsColon <@t> jmmc.com>
Subject: RE: [Histonet] Stain for HP
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <E361E3E93C280646ABBF3F8374A5874404AE8436 <@t> Exchange01.jmhosp.org>
Content-Type: text/plain;       charset="us-ascii"

Routinely, manual Diff-Quik on all gastric biopsies as well as by
request.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Diana
McCaig
Sent: Wednesday, October 17, 2012 11:04 AM
To: Histonet
Subject: [Histonet] Stain for HP

What stain are you using for HP?--Giemsa. Warthin Starry or IHC.
Do you do them routinely or only when requested?
Are they done on an autostainer or with a kit?

Diana

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notify us immediately by replying to the message and deleting it from your computer.

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Message: 13
Date: Wed, 17 Oct 2012 16:01:23 +0000
From: Remy Lopez <remy.lopez <@t> applieddiagnostics.com>
Subject: [Histonet] Job opening in Houston, Texas
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <B7FA17A269BD614984F29AB4ADBD8B8C697634 <@t> ORD2MBX02E.mex05.mlsrvr.com>
Content-Type: text/plain; charset="us-ascii"

Position:  Histotechnologist

Location:  Houston, Texas

Shift:  Monday-Friday Day Shift

To be considered email an updated resume to

Laura Vanderzyden

Human Resources Manager

Houston, Texas

TEL: (713) 271-6881

Laura.vanderzyden <@t> applieddiagnostics.com<mailto:Laura.vanderzyden <@t> applieddiagnostics.com>

------------------------------

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