[Histonet] protein concentration on tissue culture supernatant

Thurby, Christina christina.thurby <@t> bms.com
Thu Oct 11 14:05:39 CDT 2012

Please don't blast me if this is a really dumb question.  I have a tissue culture supernatant antibody.  I need to know the protein concentration - called the vendor, it is unknown.  Does anyone know if I can just run a Bradford to get the protein concentration - or will it be irrelevant do to other proteins present in the antibody?  This is an IVD antibody, so I expect it to be specific for the target antigen.  This is an unconjugated antibody and I want to biotinylate it, but I need a starting protein concentration.  Anyone out there with experience doing this with a tissue culture supernatant antibody?

Christina Thurby
Bristol Myers Squibb

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