[Histonet] help with dead fungi

[Rene J Buesa]

Do you mean to try to demonstrate if the fungi you find in a sample WAS dead or alive before it was "killed" during the fixation and processing?
My take on this is the following: if a fungi "dies" naturally in a tissue (either lung or nail, or whatever) that dead fungi either is decayed and lost or its shape has to be modified in a way that it can be microscopically distinguished by the pathologist from other "alive" fungi.
On the other hand you could try to use DNA or RNA staining to localize the nuclei and decide if the structure "seems to correspond" to that of an alive fungi.
I think that if you find fungal structures in abundance in a FFPE tissue they should correspond to living fungi at the moment the FFPE process started.
René J.


________________________________
From: "Bartlett, Jeanine (CDC/OID/NCEZID)" <jqb7 <@t> cdc.gov>
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Thursday, October 11, 2012 7:53 AM
Subject: [Histonet] help with dead fungi

Good morning everyone,

Does anyone have  a protocol they use to demonstrate dead fungi in FFPPE tissue?

Thanks!

Jeanine H. Bartlett, BS HT(ASCP), QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, NE
MS/G-32
Atlanta, Ga 30333
404-639-3590
jeanine.bartlett <@t> cdc.hhs.gov

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