[Histonet] immunohistochemical staining of macrophages in rat mesentery adipose tissue reg

[Elizabeth Chlipala]

Serotec ED-1 mouse anti-rat CD68 macrophage marker, we use proteinase K for retrieval.  You need to use a secondary that is rat absorbed we use one from dako.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of abijag
Sent: Thursday, October 11, 2012 8:37 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] immunohistochemical staining of macrophages in rat mesentery adipose tissue reg

Dear Histonetters,

I am requesting your valuable advice/suggestion on immunohistochemical staining of macrophages in rat mesentery adipose tissue. I would like to know the type of primary and secondary antibodies you are using(manufacturer details)and your preferable protocol etc.



Thanks in advance



Abi Jagannath
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